Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation

Takashi Kubota, Yuki Katou, Ryuichiro Nakato, Katsuhiko Shirahige, Anne Dunlop Donaldson

Research output: Contribution to journalArticle

35 Citations (Scopus)
8 Downloads (Pure)

Abstract

The sliding clamp PCNA is a crucial component of the DNA replication machinery. Timely PCNA loading and unloading are central for genome integrity and must be strictly coordinated with other DNA processing steps during replication. Here, we show that the S. cerevisiae Elg1 replication factor C-like complex (Elg1-RLC) unloads PCNA genome-wide following Okazaki fragment ligation. In the absence of Elg1, PCNA is retained on chromosomes in the wake of replication forks, rather than at specific sites. Degradation of the Okazaki fragment ligase Cdc9 leads to PCNA accumulation on chromatin, similar to the accumulation caused by lack of Elg1. We demonstrate that Okazaki fragment ligation is the critical prerequisite for PCNA unloading, since Chlorella virus DNA ligase can substitute for Cdc9 in yeast and simultaneously promotes PCNA unloading. Our results suggest that Elg1-RLC acts as a general PCNA unloader and is dependent upon DNA ligation during chromosome replication.
Original languageEnglish
Pages (from-to)774-787
Number of pages14
JournalCell Reports
Volume12
Issue number5
Early online date23 Jul 2015
DOIs
Publication statusPublished - 4 Aug 2015

Fingerprint Dive into the research topics of 'Replication-Coupled PCNA Unloading by the Elg1 Complex Occurs Genome-wide and Requires Okazaki Fragment Ligation'. Together they form a unique fingerprint.

  • Cite this