Reporting, appraising, and integrating data on genotype prevalence and gene-disease associations

Julian Little, L. Bradley, M. Clyne, J. Hanson, M. Khoury, J. Lau, T. R. O'Brien, N. Rothman, D. Stroup, E. Taioli, M. S. Bray, J. Dorman, DL. Ellsworth

    Research output: Contribution to journalArticle

    326 Citations (Scopus)

    Abstract

    The recent completion of the first draft of the human genome sequence and advances in technologies for genomic analysis are generating tremendous opportunities for epidemiologic studies to evaluate the role of genetic variants in human disease. Many methodological issues apply to the investigation of variation in the frequency of allelic variants of human genes, of the possibility that these influence disease risk, and of assessment of the magnitude of the associated risk. Based on a Human Genome Epidemiology workshop, a checklist for reporting and appraising studies of genotype prevalence and studies of gene-disease associations was developed. This focuses on selection of study subjects, analytic validity of genotyping, population stratification, and statistical issues. Use of the checklist should facilitate the integration of evidence from these studies. The relation between the checklist and grading schemes that have been proposed for the evaluation of observational studies is discussed. Although the limitations of grading schemes are recognized, a robust approach is proposed. Other issues in the synthesis of evidence that are particularly relevant to studies of genotype prevalence and gene-disease association are discussed, notably identification of studies, publication bias, criteria for causal inference, and the appropriateness of quantitative synthesis.

    Original languageEnglish
    Pages (from-to)300-310
    Number of pages10
    JournalAmerican Journal of Epidemiology
    Volume156
    Issue number4
    DOIs
    Publication statusPublished - 2002

    Keywords

    • case-control studies
    • causality
    • cohort studies
    • epidemlologic methods
    • gene frequency
    • genetic techniques
    • meta-analysis
    • SINGLE NUCLEOTIDE POLYMORPHISMS
    • COMPLEX HUMAN-DISEASES
    • HUMAN GENOME
    • COLORECTAL-CANCER
    • POPULATION STRATIFICATION
    • LINKAGE DISEQUILIBRIUM
    • DIABETES-MELLITUS
    • POOLED ANALYSES
    • BLOOD SPOTS
    • METAANALYSIS

    Cite this

    Reporting, appraising, and integrating data on genotype prevalence and gene-disease associations. / Little, Julian; Bradley, L.; Clyne, M.; Hanson, J.; Khoury, M.; Lau, J.; O'Brien, T. R.; Rothman, N.; Stroup, D.; Taioli, E.; Bray, M. S.; Dorman, J.; Ellsworth, DL.

    In: American Journal of Epidemiology, Vol. 156, No. 4, 2002, p. 300-310.

    Research output: Contribution to journalArticle

    Little, J, Bradley, L, Clyne, M, Hanson, J, Khoury, M, Lau, J, O'Brien, TR, Rothman, N, Stroup, D, Taioli, E, Bray, MS, Dorman, J & Ellsworth, DL 2002, 'Reporting, appraising, and integrating data on genotype prevalence and gene-disease associations', American Journal of Epidemiology, vol. 156, no. 4, pp. 300-310. https://doi.org/10.1093/aje/kwf054
    Little, Julian ; Bradley, L. ; Clyne, M. ; Hanson, J. ; Khoury, M. ; Lau, J. ; O'Brien, T. R. ; Rothman, N. ; Stroup, D. ; Taioli, E. ; Bray, M. S. ; Dorman, J. ; Ellsworth, DL. / Reporting, appraising, and integrating data on genotype prevalence and gene-disease associations. In: American Journal of Epidemiology. 2002 ; Vol. 156, No. 4. pp. 300-310.
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