Requirement for the Rac GTPase in Chlamydia trachomatis invasion of non-phagocytic cells

Rey A Carabeo, Scott S Grieshaber, Aaron Hasenkrug, Cheryl Dooley, Ted Hackstadt

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Chlamydiae are gram-negative obligate intracellular pathogens to which access to an intracellular environment is paramount to their survival and replication. To this end, chlamydiae have evolved extremely efficient means of invading nonphagocytic cells. To elucidate the host cell machinery utilized by Chlamydia trachomatis in invasion, we examined the roles of the Rho GTPase family members in the internalization of chlamydial elementary bodies. Upon binding of elementary bodies on the cell surface, actin is rapidly recruited to the sites of internalization. Members of the Rho GTPase family are frequently involved in localized recruitment of actin. Clostridial Toxin B, which is a known enzymatic inhibitor of Rac, Cdc42 and Rho GTPases, significantly reduced chlamydial invasion of HeLa cells. Expression of dominant negative constructs in HeLa cells revealed that chlamydial uptake was dependent on Rac, but not on Cdc42 or RhoA. Rac but not Cdc42 was found to be activated by chlamydial attachment. The effect of dominant negative Rac expression on chlamydial uptake is manifested through the inhibition of actin recruitment to the sites of chlamydial entry. Studies utilizing Green Fluorescent Protein fusion constructs of Rac, Cdc42 and RhoA, showed Rac to be the sole member of the Rho GTPase family recruited to the site of chlamydial entry.
Original languageEnglish
Pages (from-to)418-25
Number of pages8
JournalTraffic
Volume5
Issue number6
DOIs
Publication statusPublished - Jun 2004

Fingerprint

rho GTP-Binding Proteins
GTP Phosphohydrolases
Chlamydia trachomatis
Actins
Chlamydia
HeLa Cells
Pathogens
Green Fluorescent Proteins
Machinery
Fusion reactions

Keywords

  • Bacterial Toxins
  • Microscopy, Confocal
  • Animals
  • Enzyme Activation
  • HeLa Cells
  • Green Fluorescent Proteins
  • Humans
  • Chlamydia trachomatis
  • Recombinant Fusion Proteins
  • Enzyme Inhibitors
  • Plasmids
  • Genes, Dominant
  • Actins
  • rac GTP-Binding Proteins
  • Phagocytosis

Cite this

Carabeo, R. A., Grieshaber, S. S., Hasenkrug, A., Dooley, C., & Hackstadt, T. (2004). Requirement for the Rac GTPase in Chlamydia trachomatis invasion of non-phagocytic cells. Traffic, 5(6), 418-25. https://doi.org/10.1111/j.1398-9219.2004.00184.x

Requirement for the Rac GTPase in Chlamydia trachomatis invasion of non-phagocytic cells. / Carabeo, Rey A; Grieshaber, Scott S; Hasenkrug, Aaron; Dooley, Cheryl; Hackstadt, Ted.

In: Traffic, Vol. 5, No. 6, 06.2004, p. 418-25.

Research output: Contribution to journalArticle

Carabeo, RA, Grieshaber, SS, Hasenkrug, A, Dooley, C & Hackstadt, T 2004, 'Requirement for the Rac GTPase in Chlamydia trachomatis invasion of non-phagocytic cells', Traffic, vol. 5, no. 6, pp. 418-25. https://doi.org/10.1111/j.1398-9219.2004.00184.x
Carabeo, Rey A ; Grieshaber, Scott S ; Hasenkrug, Aaron ; Dooley, Cheryl ; Hackstadt, Ted. / Requirement for the Rac GTPase in Chlamydia trachomatis invasion of non-phagocytic cells. In: Traffic. 2004 ; Vol. 5, No. 6. pp. 418-25.
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