Response of a Rhizobium-based luminescence biosensor to Zn and Cu in soil solutions from sewage sludge treated soils

A M Chaudri, K Lawlor, S Preston, G I Paton, K Killham, S P McGrath

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37 Citations (Scopus)

Abstract

A luminescence based biosensor (Rhizotox-C) was used as an indicator of heavy metal pollution of soils. The response of the biosensor to increasing concentrations of total soil Zn, soil solution Zn, soil solution free Zn2+, total soil Cu and total soil solution Cu from soils of a long-term sewage sludge field experiment was investigated. The bioluminescence response of the Rhizotox-C biosensor declined as total soil Zn, soil solution Zn and free soil solution Zn2+ concentrations increased. The EC25 values for the biosensor for total soil Zn, soluble soil solution Zn and free soil solution Zn2+ were 164 +/- 43 mg kg(-1) soil, 4 +/- 0.7 and 2 +/- 0.3 mg 1(-1), respectively. The EC50 values were 403 +/- 57 mg kg(-1) soil, 16 +/- 3 and 6 +/- 1.0 mg 1(-1), respectively. The largest soil solution concentration of Cu was about 620 mu g 1(-1), but this had no significant effect on luminescence. This corresponded to a total Cu concentration in bulk soil of about 349 mg kg(-1). (C) 2000 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)383-388
Number of pages6
JournalSoil Biology and Biochemistry
Volume32
Publication statusPublished - 2000

Keywords

  • LEGUMINOSARUM BIOVAR TRIFOLII
  • MICROBIAL BIOMASS
  • METALS
  • MICROORGANISMS
  • TOXICITY
  • CADMIUM
  • ZINC
  • SPECIATION
  • CD
  • NI

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