A luminescence based biosensor (Rhizotox-C) was used as an indicator of heavy metal pollution of soils. The response of the biosensor to increasing concentrations of total soil Zn, soil solution Zn, soil solution free Zn2+, total soil Cu and total soil solution Cu from soils of a long-term sewage sludge field experiment was investigated. The bioluminescence response of the Rhizotox-C biosensor declined as total soil Zn, soil solution Zn and free soil solution Zn2+ concentrations increased. The EC25 values for the biosensor for total soil Zn, soluble soil solution Zn and free soil solution Zn2+ were 164 +/- 43 mg kg(-1) soil, 4 +/- 0.7 and 2 +/- 0.3 mg 1(-1), respectively. The EC50 values were 403 +/- 57 mg kg(-1) soil, 16 +/- 3 and 6 +/- 1.0 mg 1(-1), respectively. The largest soil solution concentration of Cu was about 620 mu g 1(-1), but this had no significant effect on luminescence. This corresponded to a total Cu concentration in bulk soil of about 349 mg kg(-1). (C) 2000 Elsevier Science Ltd. All rights reserved.
|Number of pages||6|
|Journal||Soil Biology and Biochemistry|
|Publication status||Published - 2000|
- LEGUMINOSARUM BIOVAR TRIFOLII
- MICROBIAL BIOMASS