Response of apolipoprotein E*3-Leiden transgenic mice to dietary fatty acids: combining liver proteomics with physiological data

Baukje De Roos, Ilse Duivenvoorden, Garry Jonathan Rucklidge, Martin David Reid, Karen Ross, Robert-Jan A N Lamers, Peter J Voshol, Louis M Havekes, Bas Teusink

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    Dietary fatty acids have a profound impact on atherosclerosis, but mechanisms are not fully understood. We studied the effects of a saturated fat diet supplemented with fish oil, trans10,cis12 conjugated linoleic acid (CLA), or elaidic acid on lipid and glucose metabolism and liver protein levels of APOE*3 Leiden transgenic mice, a model for lipid metabolism and atherosclerosis. Fish oil lowered plasma and liver cholesterol and triglycerides, plasma free fatty acids, and glucose but increased plasma insulin. CLA lowered plasma cholesterol but increased plasma and liver triglycerides, plasma beta-hydroxybutyrate, and insulin. Elaidic acid lowered plasma and liver cholesterol. Proteomics identified significant regulation of 65 cytosolic and 8-membrane proteins. Many of these proteins were related to lipid and glucose metabolism, and to oxidative stress. Principal component analysis revealed that fish oil had a major impact on cytosolic proteins, and elaidic acid on membrane proteins. Correlation analysis between physiological and protein data revealed novel clusters of correlated variables, among which a metabolic syndrome cluster. The combination of proteomics and physiology gave new insights in mechanisms by which these dietary fatty acids regulate lipid metabolism and related pathways, for example, by altering protein levels of long-chain acyl-CoA thioester hydrolase and adipophilin in the liver.
    Original languageEnglish
    Pages (from-to)813-815
    Number of pages3
    JournalThe FASEB Journal
    Volume19
    Issue number7
    Early online date8 Mar 2005
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Liver
    Proteomics
    Transgenic Mice
    Fatty Acids
    Plasmas
    Lipid Metabolism
    Fish Oils
    Conjugated Linoleic Acids
    Cholesterol
    Proteins
    Metabolism
    Glucose
    Atherosclerosis
    Membrane Proteins
    Triglycerides
    Palmitoyl-CoA Hydrolase
    Insulin
    Lipids
    Acyl Coenzyme A
    Oxidative stress

    Keywords

    • 3-Hydroxybutyric Acid
    • Animals
    • Apolipoprotein E3
    • Apolipoproteins E
    • Atherosclerosis
    • Blood Glucose
    • Cell Membrane
    • Cholesterol
    • Cytosol
    • Dietary Fats
    • Disease Models, Animal
    • Electrophoresis, Gel, Two-Dimensional
    • Fatty Acids
    • Female
    • Fish Oils
    • Insulin
    • Linoleic Acids, Conjugated
    • Lipid Metabolism
    • Lipids
    • Liver
    • Mice
    • Mice, Transgenic
    • Oleic Acid
    • Organ Size
    • Proteins
    • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
    • Triglycerides
    • Fish Oil
    • CLA
    • Elaidic acid
    • Lipoprotein metabolism
    • Glucose metabolism

    Cite this

    Response of apolipoprotein E*3-Leiden transgenic mice to dietary fatty acids : combining liver proteomics with physiological data. / De Roos, Baukje; Duivenvoorden, Ilse; Rucklidge, Garry Jonathan; Reid, Martin David; Ross, Karen; Lamers, Robert-Jan A N; Voshol, Peter J; Havekes, Louis M; Teusink, Bas.

    In: The FASEB Journal, Vol. 19, No. 7, 2005, p. 813-815.

    Research output: Contribution to journalArticle

    De Roos, B, Duivenvoorden, I, Rucklidge, GJ, Reid, MD, Ross, K, Lamers, R-JAN, Voshol, PJ, Havekes, LM & Teusink, B 2005, 'Response of apolipoprotein E*3-Leiden transgenic mice to dietary fatty acids: combining liver proteomics with physiological data', The FASEB Journal, vol. 19, no. 7, pp. 813-815. https://doi.org/10.1096/fj.04-2974fje
    De Roos, Baukje ; Duivenvoorden, Ilse ; Rucklidge, Garry Jonathan ; Reid, Martin David ; Ross, Karen ; Lamers, Robert-Jan A N ; Voshol, Peter J ; Havekes, Louis M ; Teusink, Bas. / Response of apolipoprotein E*3-Leiden transgenic mice to dietary fatty acids : combining liver proteomics with physiological data. In: The FASEB Journal. 2005 ; Vol. 19, No. 7. pp. 813-815.
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    T2 - combining liver proteomics with physiological data

    AU - De Roos, Baukje

    AU - Duivenvoorden, Ilse

    AU - Rucklidge, Garry Jonathan

    AU - Reid, Martin David

    AU - Ross, Karen

    AU - Lamers, Robert-Jan A N

    AU - Voshol, Peter J

    AU - Havekes, Louis M

    AU - Teusink, Bas

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    N2 - Dietary fatty acids have a profound impact on atherosclerosis, but mechanisms are not fully understood. We studied the effects of a saturated fat diet supplemented with fish oil, trans10,cis12 conjugated linoleic acid (CLA), or elaidic acid on lipid and glucose metabolism and liver protein levels of APOE*3 Leiden transgenic mice, a model for lipid metabolism and atherosclerosis. Fish oil lowered plasma and liver cholesterol and triglycerides, plasma free fatty acids, and glucose but increased plasma insulin. CLA lowered plasma cholesterol but increased plasma and liver triglycerides, plasma beta-hydroxybutyrate, and insulin. Elaidic acid lowered plasma and liver cholesterol. Proteomics identified significant regulation of 65 cytosolic and 8-membrane proteins. Many of these proteins were related to lipid and glucose metabolism, and to oxidative stress. Principal component analysis revealed that fish oil had a major impact on cytosolic proteins, and elaidic acid on membrane proteins. Correlation analysis between physiological and protein data revealed novel clusters of correlated variables, among which a metabolic syndrome cluster. The combination of proteomics and physiology gave new insights in mechanisms by which these dietary fatty acids regulate lipid metabolism and related pathways, for example, by altering protein levels of long-chain acyl-CoA thioester hydrolase and adipophilin in the liver.

    AB - Dietary fatty acids have a profound impact on atherosclerosis, but mechanisms are not fully understood. We studied the effects of a saturated fat diet supplemented with fish oil, trans10,cis12 conjugated linoleic acid (CLA), or elaidic acid on lipid and glucose metabolism and liver protein levels of APOE*3 Leiden transgenic mice, a model for lipid metabolism and atherosclerosis. Fish oil lowered plasma and liver cholesterol and triglycerides, plasma free fatty acids, and glucose but increased plasma insulin. CLA lowered plasma cholesterol but increased plasma and liver triglycerides, plasma beta-hydroxybutyrate, and insulin. Elaidic acid lowered plasma and liver cholesterol. Proteomics identified significant regulation of 65 cytosolic and 8-membrane proteins. Many of these proteins were related to lipid and glucose metabolism, and to oxidative stress. Principal component analysis revealed that fish oil had a major impact on cytosolic proteins, and elaidic acid on membrane proteins. Correlation analysis between physiological and protein data revealed novel clusters of correlated variables, among which a metabolic syndrome cluster. The combination of proteomics and physiology gave new insights in mechanisms by which these dietary fatty acids regulate lipid metabolism and related pathways, for example, by altering protein levels of long-chain acyl-CoA thioester hydrolase and adipophilin in the liver.

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    KW - Cholesterol

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    KW - Electrophoresis, Gel, Two-Dimensional

    KW - Fatty Acids

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    KW - Mice, Transgenic

    KW - Oleic Acid

    KW - Organ Size

    KW - Proteins

    KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

    KW - Triglycerides

    KW - Fish Oil

    KW - CLA

    KW - Elaidic acid

    KW - Lipoprotein metabolism

    KW - Glucose metabolism

    U2 - 10.1096/fj.04-2974fje

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    VL - 19

    SP - 813

    EP - 815

    JO - The FASEB Journal

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