Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

TY - JOUR

T1 - Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

AU - Hoskin, S. O.

AU - Bremner, D. M.

AU - Holtrop, G.

AU - Lobley, G. E.

N1 - Acknowledgements The expertise of A. Graham Calder and Susan Anderson for the various stable isotope analyses is gratefully recognised. Celine Germaine performed the SDH measurements from the tissue samples as part of a student placement from AgroParisTech, Paris, France. This study was funded as part of the core grant provided to the Rowett Institute of Nutrition and Health, University of Aberdeen and Biomathematics and Statistics Scotland by Rural and Environment Science and Analytical Services, part of the Scottish Government. Hoskin was a recipient of a FoRST (NZ) award to study abroad. The authors’ contributions were as follows: S. O. H. and G. E. L. were responsible for study concept and design. S. O. H., G. E. L. and D. M. B. were responsible for data collection and collation. G. E. L. and G. H. were responsible for data analysis and statistical matters. S. O. H., G. E. L. and G. H. were responsible for the first draft and critical revision of the manuscript for important intellectual content. The authors declare that there are no conflicts of interest

PY - 2016/2/28

Y1 - 2016/2/28

N2 - Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P

AB - Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P

KW - endotoxin lipopolysaccharide

KW - amino acids

KW - irreversible loss rate

KW - serine-threonine dehydratase

KW - sheep

KW - acute-phase proteins

KW - teladorsagia-circumcincta

KW - porcine model

KW - septic rats

KW - metabolism

KW - sepsis

KW - arginine

KW - plasma

KW - infection

U2 - 10.1017/S0007114515004894

DO - 10.1017/S0007114515004894

M3 - Article

VL - 115

SP - 576

EP - 584

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 4

ER -