Responses in whole-body amino acid kinetics to an acute, sub-clinical endotoxin challenge in lambs

S. O. Hoskin, D. M. Bremner, G. Holtrop, G. E. Lobley*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Some effects of parasitism, endotoxaemia or sepsis can be mitigated by provision of extra protein. Supplemented protein may encompass a metabolic requirement for specific amino acids (AA). The current study investigates a method to identify and quantify the amounts of AA required during inflammation induced by an endotoxin challenge. One of each pair of six twin sheep was infused in the jugular vein for 20 h with either saline (control) or lipopolysaccharide (LPS, 2 ng/kg body weight per min) from Escherichia coli. Between 12 and 20 h a mixture of stable isotope-labelled AA was infused to measure irreversible loss rates. From 16 to 20 h all sheep were supplemented with a mixture of unlabelled AA infused intravenously. Blood samples were taken before the start of infusions, and then continuously over intervals between 14 and 20 h. At 20 h the sheep were euthanised, and liver and kidney samples were taken for measurement of serine-threonine dehydratase (SDH) activity. LPS infusion decreased plasma concentrations of most AA (P

Original languageEnglish
Pages (from-to)576-584
Number of pages9
JournalBritish Journal of Nutrition
Volume115
Issue number4
Early online date14 Dec 2015
DOIs
Publication statusPublished - 28 Feb 2016

Keywords

  • endotoxin lipopolysaccharide
  • amino acids
  • irreversible loss rate
  • serine-threonine dehydratase
  • sheep
  • acute-phase proteins
  • teladorsagia-circumcincta
  • porcine model
  • septic rats
  • metabolism
  • sepsis
  • arginine
  • plasma
  • infection

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