Retinal ganglion cell axon guidance in the mouse optic chiasm: Expression and Function of Robos and Slits

Lynda Erskine, S E Williams, K Brose, T Kidd, R A Rachel, C S Goodman, M Tessier-Lavigne, C A Mason

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

The ventral midline of the nervous system is an important choice point at which growing axons decide whether to cross and project contralaterally or remain on the same side of the brain. In Drosophila, the decision to cross or avoid the CNS midline is controlled, at least in part, by the Roundabout (Robo) receptor on the axons and its ligand, Slit, an inhibitory extracellular matrix molecule secreted by the midline glia. Vertebrate homologs of these molecules have been cloned and have also been implicated in regulating axon guidance. Using in situ hybridization, we have determined the expression patterns of robo1,2 and slit1,2,3 in the mouse retina and in the region of the developing optic chiasm, a ventral midline structure in which retinal ganglion cell (RGC) axons diverge to either side of the brain. The receptors and ligands are expressed at the appropriate time and place, in both the retina and the ventral diencephalon, to be able to influence RGC axon guidance. In vitro, slit2 is inhibitory to RGC axons, with outgrowth of both ipsilaterally and contralaterally projecting axons being strongly affected. Overall, these results indicate that Robos and Slits alone do not directly control RGC axon divergence at the optic chiasm and may additionally function as a general inhibitory guidance system involved in determining the relative position of the optic chiasm at the ventral midline of the developing hypothalamus.
Original languageEnglish
Pages (from-to)4975-4982
Number of pages8
JournalJournal of Neuroscience
Volume20
Issue number13
Publication statusPublished - 1 Jul 2000

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Optic Chiasm
Retinal Ganglion Cells
Axons
Retina
Ligands
Diencephalon
Brain
Neuroglia
Nervous System
Hypothalamus
Drosophila
In Situ Hybridization
Extracellular Matrix
Vertebrates
Axon Guidance

Keywords

  • Animals
  • Axons
  • Diencephalon
  • Embryonic and Fetal Development
  • Gene Expression Regulation, Developmental
  • Intercellular Signaling Peptides and Proteins
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins
  • Optic Chiasm
  • Organ Culture Techniques
  • Receptors, Immunologic
  • Retina
  • Retinal Ganglion Cells
  • axon guidance
  • diencephalon
  • hypothalamus
  • optic chiasm
  • Robo
  • retinal ganglion cell
  • slit

Cite this

Erskine, L., Williams, S. E., Brose, K., Kidd, T., Rachel, R. A., Goodman, C. S., ... Mason, C. A. (2000). Retinal ganglion cell axon guidance in the mouse optic chiasm: Expression and Function of Robos and Slits . Journal of Neuroscience, 20(13), 4975-4982.

Retinal ganglion cell axon guidance in the mouse optic chiasm : Expression and Function of Robos and Slits . / Erskine, Lynda; Williams, S E; Brose, K; Kidd, T; Rachel, R A; Goodman, C S; Tessier-Lavigne, M; Mason, C A.

In: Journal of Neuroscience, Vol. 20, No. 13, 01.07.2000, p. 4975-4982.

Research output: Contribution to journalArticle

Erskine, L, Williams, SE, Brose, K, Kidd, T, Rachel, RA, Goodman, CS, Tessier-Lavigne, M & Mason, CA 2000, 'Retinal ganglion cell axon guidance in the mouse optic chiasm: Expression and Function of Robos and Slits ', Journal of Neuroscience, vol. 20, no. 13, pp. 4975-4982.
Erskine L, Williams SE, Brose K, Kidd T, Rachel RA, Goodman CS et al. Retinal ganglion cell axon guidance in the mouse optic chiasm: Expression and Function of Robos and Slits . Journal of Neuroscience. 2000 Jul 1;20(13):4975-4982.
Erskine, Lynda ; Williams, S E ; Brose, K ; Kidd, T ; Rachel, R A ; Goodman, C S ; Tessier-Lavigne, M ; Mason, C A. / Retinal ganglion cell axon guidance in the mouse optic chiasm : Expression and Function of Robos and Slits . In: Journal of Neuroscience. 2000 ; Vol. 20, No. 13. pp. 4975-4982.
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AU - Erskine, Lynda

AU - Williams, S E

AU - Brose, K

AU - Kidd, T

AU - Rachel, R A

AU - Goodman, C S

AU - Tessier-Lavigne, M

AU - Mason, C A

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N2 - The ventral midline of the nervous system is an important choice point at which growing axons decide whether to cross and project contralaterally or remain on the same side of the brain. In Drosophila, the decision to cross or avoid the CNS midline is controlled, at least in part, by the Roundabout (Robo) receptor on the axons and its ligand, Slit, an inhibitory extracellular matrix molecule secreted by the midline glia. Vertebrate homologs of these molecules have been cloned and have also been implicated in regulating axon guidance. Using in situ hybridization, we have determined the expression patterns of robo1,2 and slit1,2,3 in the mouse retina and in the region of the developing optic chiasm, a ventral midline structure in which retinal ganglion cell (RGC) axons diverge to either side of the brain. The receptors and ligands are expressed at the appropriate time and place, in both the retina and the ventral diencephalon, to be able to influence RGC axon guidance. In vitro, slit2 is inhibitory to RGC axons, with outgrowth of both ipsilaterally and contralaterally projecting axons being strongly affected. Overall, these results indicate that Robos and Slits alone do not directly control RGC axon divergence at the optic chiasm and may additionally function as a general inhibitory guidance system involved in determining the relative position of the optic chiasm at the ventral midline of the developing hypothalamus.

AB - The ventral midline of the nervous system is an important choice point at which growing axons decide whether to cross and project contralaterally or remain on the same side of the brain. In Drosophila, the decision to cross or avoid the CNS midline is controlled, at least in part, by the Roundabout (Robo) receptor on the axons and its ligand, Slit, an inhibitory extracellular matrix molecule secreted by the midline glia. Vertebrate homologs of these molecules have been cloned and have also been implicated in regulating axon guidance. Using in situ hybridization, we have determined the expression patterns of robo1,2 and slit1,2,3 in the mouse retina and in the region of the developing optic chiasm, a ventral midline structure in which retinal ganglion cell (RGC) axons diverge to either side of the brain. The receptors and ligands are expressed at the appropriate time and place, in both the retina and the ventral diencephalon, to be able to influence RGC axon guidance. In vitro, slit2 is inhibitory to RGC axons, with outgrowth of both ipsilaterally and contralaterally projecting axons being strongly affected. Overall, these results indicate that Robos and Slits alone do not directly control RGC axon divergence at the optic chiasm and may additionally function as a general inhibitory guidance system involved in determining the relative position of the optic chiasm at the ventral midline of the developing hypothalamus.

KW - Animals

KW - Axons

KW - Diencephalon

KW - Embryonic and Fetal Development

KW - Gene Expression Regulation, Developmental

KW - Intercellular Signaling Peptides and Proteins

KW - Mice

KW - Mice, Inbred C57BL

KW - Nerve Tissue Proteins

KW - Optic Chiasm

KW - Organ Culture Techniques

KW - Receptors, Immunologic

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KW - Retinal Ganglion Cells

KW - axon guidance

KW - diencephalon

KW - hypothalamus

KW - optic chiasm

KW - Robo

KW - retinal ganglion cell

KW - slit

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JF - Journal of Neuroscience

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