Ribosome recycling induces optimal translation rate at low ribosomal availability

E. Marshall (Corresponding Author), I. Stansfield, M. C. Romano (Corresponding Author)

Research output: Contribution to journalArticle

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Abstract

During eukaryotic cellular protein synthesis, ribosomal translation is made more efficient through interaction between the two ends of the messenger RNA (mRNA). Ribosomes reaching the 3′ end of the mRNA can thus recycle and begin translation again on the same mRNA, the so-called ‘closed-loop’ model. Using a driven diffusion lattice model of translation, we study the effects of ribosome recycling on the dynamics of ribosome flow and density on the mRNA. We show that ribosome recycling induces a substantial increase in ribosome current. Furthermore, for sufficiently large values of the recycling rate, the lattice does not transition directly from low to high ribosome density, as seen in lattice models without recycling. Instead, a maximal current phase becomes accessible for much lower values of the initiation rate, and multiple phase transitions occur over a wide region of the phase plane. Crucially, we show that in the presence of ribosome recycling, mRNAs can exhibit a peak in protein production at low values of the initiation rate, beyond which translation rate decreases. This has important implications for translation of certain mRNAs, suggesting that there is an optimal concentration of ribosomes at which protein synthesis is maximal, and beyond which translational efficiency is impaired.
Original languageEnglish
Article number20140589
Number of pages9
JournalJournal of the Royal Society Interface
Volume11
Issue number98
Early online date9 Jul 2014
DOIs
Publication statusPublished - 6 Sep 2014

Fingerprint

Ribosomes
Recycling
Availability
Messenger RNA
Proteins
Phase Transition
Protein Biosynthesis
Phase transitions

Keywords

  • ribosome recycling
  • translation
  • totally asymmetric simple exclusion process
  • RLI1
  • ABCE1

Cite this

Ribosome recycling induces optimal translation rate at low ribosomal availability. / Marshall, E. (Corresponding Author); Stansfield, I.; Romano, M. C. (Corresponding Author).

In: Journal of the Royal Society Interface, Vol. 11, No. 98, 20140589 , 06.09.2014.

Research output: Contribution to journalArticle

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