Ric1, a Phytophthora infestans gene with homology to stress-induced genes

P van West, S Kamoun, J M van't Klooster, F Govers

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato-P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98% homology at the nucleotide-sequence level and 100% at the aminoacid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytopophthora species indicated that most species contain lid homologues. During the life cycle of P. infestans, rid was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.

Original languageEnglish
Pages (from-to)310-315
Number of pages6
JournalCurrent Opinion in Genetics & Development
Volume36
Publication statusPublished - 1999

Keywords

  • Phytophthora infestans
  • potato late blight
  • stress-induced gene
  • IPIO GENE
  • EXPRESSION
  • POTATO

Cite this

Ric1, a Phytophthora infestans gene with homology to stress-induced genes. / van West, P ; Kamoun, S ; van't Klooster, J M ; Govers, F .

In: Current Opinion in Genetics & Development, Vol. 36, 1999, p. 310-315.

Research output: Contribution to journalArticle

van West, P ; Kamoun, S ; van't Klooster, J M ; Govers, F . / Ric1, a Phytophthora infestans gene with homology to stress-induced genes. In: Current Opinion in Genetics & Development. 1999 ; Vol. 36. pp. 310-315.
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abstract = "From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato-P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98{\%} homology at the nucleotide-sequence level and 100{\%} at the aminoacid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytopophthora species indicated that most species contain lid homologues. During the life cycle of P. infestans, rid was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.",
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AU - van't Klooster, J M

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N2 - From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato-P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98% homology at the nucleotide-sequence level and 100% at the aminoacid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytopophthora species indicated that most species contain lid homologues. During the life cycle of P. infestans, rid was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.

AB - From a set of Phytophthora infestans cDNA clones that were randomly selected from a potato-P. infestans interaction cDNA library, a relatively high proportion (5 out of 22) appeared to be derived from the same gene. The gene was designated ric1. P. infestans contains two copies of ric1 which share 98% homology at the nucleotide-sequence level and 100% at the aminoacid level. The nucleotide sequence predicts an open reading frame of 171 bp encoding a 57 amino-acid hydrophobic-peptide with two potential membrane-spanning domains. The predicted peptide shows high homology to a peptide encoded by plant genes whose expression is specifically induced during stress conditions. Southern-blot analysis of genomic DNA of several Phytopophthora species indicated that most species contain lid homologues. During the life cycle of P. infestans, rid was expressed in all developmental stages but the level of expression varied. Sporangia and germinating cysts appeared to contain only very little ric1 mRNA whereas in the mycelium and during in planta growth higher levels were detected. Subjecting the mycelium to osmotic stress or to a high pH resulted in increased ric1 expression.

KW - Phytophthora infestans

KW - potato late blight

KW - stress-induced gene

KW - IPIO GENE

KW - EXPRESSION

KW - POTATO

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VL - 36

SP - 310

EP - 315

JO - Current Opinion in Genetics & Development

JF - Current Opinion in Genetics & Development

SN - 0959-437X

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