Rice-arsenate interactions in hydroponics: whole genome transcriptional analysis

Gareth J. Norton, Daniel E. Lou-Hing, Andrew A. Meharg, Adam H. Price

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Rice (Oryza sativa) varieties that are arsenate-tolerant (Bala) and -sensitive (Azucena) were used to conduct a transcriptome analysis of the response of rice seedlings to sodium arsenate (AsV) in hydroponic solution. RNA extracted from the roots of three replicate experiments of plants grown for 1 week in phosphate-free nutrient with or without 13.3 mu M AsV was used to challenge the Affymetrix (52K) GeneChip Rice Genome array. A total of 576 probe sets were significantly up-regulated at least 2-fold in both varieties, whereas 622 were down-regulated. Ontological classification is presented. As expected, a large number of transcription factors, stress proteins, and transporters demonstrated differential expression. Striking is the lack of response of classic oxidative stress-responsive genes or phytochelatin synthases/synthatases. However, the large number of responses from genes involved in glutathione synthesis, metabolism, and transport suggests that glutathione conjugation and arsenate methylation may be important biochemical responses to arsenate challenge. In this report, no attempt is made to dissect differences in the response of the tolerant and sensitive variety, but analysis in a companion article will link gene expression to the known tolerance loci available in the BalaxAzucena mapping population.

Original languageEnglish
Pages (from-to)2267-2276
Number of pages11
JournalJournal of Experimental Botany
Volume59
Issue number8
Early online date1 May 2008
DOIs
Publication statusPublished - 8 May 2008

Keywords

  • arsenate tolerance
  • candidate genes
  • glutathione-S-transferase
  • microarray
  • oryza sativa
  • phytochelatin synthase gene
  • glutathione transferase
  • oxidative stress
  • holcus-lanatus
  • ABC transporters
  • arabidopsis
  • arsenite
  • expression
  • tolerance
  • methyltransferase

Cite this