RNA turnover and protein synthesis in fish cells

R W Smith, R M Palmer, D F Houlihan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Protein synthesis in fish has been previously correlated with RNA content. The present study investigates whether protein and RNA synthesis rates are similarly related. Protein and RNA synthesis rates were determined from H-3-phenylalanine and H-3-uridine incorporation, respectively, and expressed as % . day(-1) and half-lives, respectively. Three fibroblast cell lines were used: BF-2, RTP, CHSE 214, which are derived from the bluegill, rainbow trout and Chinook salmon, respectively. These cells contained similar RNA concentrations (similar to 175 mu g RNA.mg(-1) cell protein). Therefore differences in protein synthesis rates, BF-2 (31.3 +/- 1.8) > RTP (25.1 +/- 1.7) > CHSE 214 (17.6 +/- 1.1), were attributable to RNA translational efficiency. The most translationally efficient RNA (BF-2 cells), 1.8 mg protein synthesised . mu g(-1) RNA . day(-1), corresponded to the lowest RNA half-life, 75.4 +/- 6.4 h. Translationally efficient RNA was also energetically efficient with BF-2 cells exploiting the least costly route of nucleotide supply (i.e. exogenous salvage) 3.5 -6.0 times more than the least translationally efficient RNA (CHSE 214 cells). These data suggest that differential nucleotide supply, between intracellular synthesis and exogenous salvage, constitutes the al-ca of pre-translational flexibility exploited to maintain RNA synthesis as a fixed energetic cost component of protein synthesis.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalJournal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Volume170
Publication statusPublished - 2000

Keywords

  • RNA half-life
  • nucleotide salvage
  • RNA labelling kinetics
  • RNA translational efficiency
  • TROUT ONCORHYNCHUS-MYKISS
  • PLASMA-MEMBRANE VESICLES
  • CULTURED MAMMALIAN-CELLS
  • RAINBOW-TROUT
  • NUCLEOSIDE TRANSPORT
  • PYRIMIDINE NUCLEOSIDES
  • ISOLATED HEPATOCYTES
  • RENAL HYPERTROPHY
  • NUCLEOTIDE POOLS
  • SKELETAL-MUSCLE

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