RNA turnover and protein synthesis in fish cells

R W Smith, R M Palmer, D F Houlihan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Protein synthesis in fish has been previously correlated with RNA content. The present study investigates whether protein and RNA synthesis rates are similarly related. Protein and RNA synthesis rates were determined from H-3-phenylalanine and H-3-uridine incorporation, respectively, and expressed as % . day(-1) and half-lives, respectively. Three fibroblast cell lines were used: BF-2, RTP, CHSE 214, which are derived from the bluegill, rainbow trout and Chinook salmon, respectively. These cells contained similar RNA concentrations (similar to 175 mu g RNA.mg(-1) cell protein). Therefore differences in protein synthesis rates, BF-2 (31.3 +/- 1.8) > RTP (25.1 +/- 1.7) > CHSE 214 (17.6 +/- 1.1), were attributable to RNA translational efficiency. The most translationally efficient RNA (BF-2 cells), 1.8 mg protein synthesised . mu g(-1) RNA . day(-1), corresponded to the lowest RNA half-life, 75.4 +/- 6.4 h. Translationally efficient RNA was also energetically efficient with BF-2 cells exploiting the least costly route of nucleotide supply (i.e. exogenous salvage) 3.5 -6.0 times more than the least translationally efficient RNA (CHSE 214 cells). These data suggest that differential nucleotide supply, between intracellular synthesis and exogenous salvage, constitutes the al-ca of pre-translational flexibility exploited to maintain RNA synthesis as a fixed energetic cost component of protein synthesis.

Original languageEnglish
Pages (from-to)135-144
Number of pages10
JournalJournal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Volume170
Publication statusPublished - 2000

Keywords

  • RNA half-life
  • nucleotide salvage
  • RNA labelling kinetics
  • RNA translational efficiency
  • TROUT ONCORHYNCHUS-MYKISS
  • PLASMA-MEMBRANE VESICLES
  • CULTURED MAMMALIAN-CELLS
  • RAINBOW-TROUT
  • NUCLEOSIDE TRANSPORT
  • PYRIMIDINE NUCLEOSIDES
  • ISOLATED HEPATOCYTES
  • RENAL HYPERTROPHY
  • NUCLEOTIDE POOLS
  • SKELETAL-MUSCLE

Cite this

RNA turnover and protein synthesis in fish cells. / Smith, R W ; Palmer, R M ; Houlihan, D F .

In: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, Vol. 170, 2000, p. 135-144.

Research output: Contribution to journalArticle

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T1 - RNA turnover and protein synthesis in fish cells

AU - Smith, R W

AU - Palmer, R M

AU - Houlihan, D F

PY - 2000

Y1 - 2000

N2 - Protein synthesis in fish has been previously correlated with RNA content. The present study investigates whether protein and RNA synthesis rates are similarly related. Protein and RNA synthesis rates were determined from H-3-phenylalanine and H-3-uridine incorporation, respectively, and expressed as % . day(-1) and half-lives, respectively. Three fibroblast cell lines were used: BF-2, RTP, CHSE 214, which are derived from the bluegill, rainbow trout and Chinook salmon, respectively. These cells contained similar RNA concentrations (similar to 175 mu g RNA.mg(-1) cell protein). Therefore differences in protein synthesis rates, BF-2 (31.3 +/- 1.8) > RTP (25.1 +/- 1.7) > CHSE 214 (17.6 +/- 1.1), were attributable to RNA translational efficiency. The most translationally efficient RNA (BF-2 cells), 1.8 mg protein synthesised . mu g(-1) RNA . day(-1), corresponded to the lowest RNA half-life, 75.4 +/- 6.4 h. Translationally efficient RNA was also energetically efficient with BF-2 cells exploiting the least costly route of nucleotide supply (i.e. exogenous salvage) 3.5 -6.0 times more than the least translationally efficient RNA (CHSE 214 cells). These data suggest that differential nucleotide supply, between intracellular synthesis and exogenous salvage, constitutes the al-ca of pre-translational flexibility exploited to maintain RNA synthesis as a fixed energetic cost component of protein synthesis.

AB - Protein synthesis in fish has been previously correlated with RNA content. The present study investigates whether protein and RNA synthesis rates are similarly related. Protein and RNA synthesis rates were determined from H-3-phenylalanine and H-3-uridine incorporation, respectively, and expressed as % . day(-1) and half-lives, respectively. Three fibroblast cell lines were used: BF-2, RTP, CHSE 214, which are derived from the bluegill, rainbow trout and Chinook salmon, respectively. These cells contained similar RNA concentrations (similar to 175 mu g RNA.mg(-1) cell protein). Therefore differences in protein synthesis rates, BF-2 (31.3 +/- 1.8) > RTP (25.1 +/- 1.7) > CHSE 214 (17.6 +/- 1.1), were attributable to RNA translational efficiency. The most translationally efficient RNA (BF-2 cells), 1.8 mg protein synthesised . mu g(-1) RNA . day(-1), corresponded to the lowest RNA half-life, 75.4 +/- 6.4 h. Translationally efficient RNA was also energetically efficient with BF-2 cells exploiting the least costly route of nucleotide supply (i.e. exogenous salvage) 3.5 -6.0 times more than the least translationally efficient RNA (CHSE 214 cells). These data suggest that differential nucleotide supply, between intracellular synthesis and exogenous salvage, constitutes the al-ca of pre-translational flexibility exploited to maintain RNA synthesis as a fixed energetic cost component of protein synthesis.

KW - RNA half-life

KW - nucleotide salvage

KW - RNA labelling kinetics

KW - RNA translational efficiency

KW - TROUT ONCORHYNCHUS-MYKISS

KW - PLASMA-MEMBRANE VESICLES

KW - CULTURED MAMMALIAN-CELLS

KW - RAINBOW-TROUT

KW - NUCLEOSIDE TRANSPORT

KW - PYRIMIDINE NUCLEOSIDES

KW - ISOLATED HEPATOCYTES

KW - RENAL HYPERTROPHY

KW - NUCLEOTIDE POOLS

KW - SKELETAL-MUSCLE

M3 - Article

VL - 170

SP - 135

EP - 144

JO - Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology

SN - 0174-1578

ER -