Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

Vanessa Durand; Joanne MacKenzie; Joel de Leon; Circe Mesa; Valerie Quesniaux; Maria Montoya; Agnes Le Bon; Simon Y C Wong

doi:10.1016/j.vaccine.2009.01.109

Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

Vanessa Durand, Joanne MacKenzie, Joel de Leon, Circe Mesa, Valerie Quesniaux, Maria Montoya, Agnes Le Bon, Simon Y C Wong

Applied Medicine

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN¿-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.

Original language	English
Pages (from-to)	1912-1922
Number of pages	11
Journal	Vaccine
Volume	27
Issue number	13
Early online date	31 Jan 2009
DOIs	https://doi.org/10.1016/j.vaccine.2009.01.109
Publication status	Published - 18 Mar 2009

Keywords

lipopolysaccharide
meningococcal disease
cross-priming

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2009.01.109

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title = "Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles",

abstract = "We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN¿-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.",

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T1 - Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

AU - Durand, Vanessa

AU - MacKenzie, Joanne

AU - de Leon, Joel

AU - Mesa, Circe

AU - Quesniaux, Valerie

AU - Montoya, Maria

AU - Le Bon, Agnes

AU - Wong, Simon Y C

PY - 2009/3/18

Y1 - 2009/3/18

N2 - We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN¿-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.

AB - We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN¿-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.

KW - lipopolysaccharide

KW - meningococcal disease

KW - cross-priming

U2 - 10.1016/j.vaccine.2009.01.109

DO - 10.1016/j.vaccine.2009.01.109

M3 - Article

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SP - 1912

EP - 1922

JO - Vaccine

JF - Vaccine

IS - 13

ER -

Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

Abstract

Keywords

UN SDGs

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