Role of lipopolysaccharide in the induction of type I interferon-dependent cross-priming and IL-10 production in mice by meningococcal outer membrane vesicles

Vanessa Durand, Joanne MacKenzie, Joel de Leon, Circe Mesa, Valerie Quesniaux, Maria Montoya, Agnes Le Bon, Simon Y C Wong

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18 Citations (Scopus)

Abstract

We investigated the contribution of lipopolysaccharide (LPS) to adjuvant properties of native outer membrane vesicles (NOMV), a vaccine candidate for meningococcal B disease. NOMV induce the maturation of and cytokine production by murine bone marrow-derived dendritic cells through both toll-like receptors (TLR) 2 and 4 which are mostly dependent on the signalling adaptor MyD88. NOMV are also able to induce B cell proliferation in splenocytes from LPS-hyporesponsive mice. However, induction of IL-10 and type I interferon-dependent, antigen-specific and IFN¿-secreting CD8+ cytotoxic T lymphocyte responses in vivo by NOMV requires LPS. The importance of LPS in the induction of IL-10 and functional cross-priming has implications for NOMV-based vaccine and adjuvant development.
Original languageEnglish
Pages (from-to)1912-1922
Number of pages11
JournalVaccine
Volume27
Issue number13
Early online date31 Jan 2009
DOIs
Publication statusPublished - 18 Mar 2009

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Keywords

  • lipopolysaccharide
  • meningococcal disease
  • cross-priming

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