Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans.

Brice Patrick Enjalbert, D. A. Smith, M. J. Cornell, I. Alam, Susan Melanie Nicholls, Alistair James Petersen Brown, J. Quinn

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

The resistance of Candida albicans to many stresses is dependent on the stress-activated protein kinase (SAPK) Hog1. Hence we have explored the role of Hog1 in the regulation of transcriptional responses to stress. DNA microarrays were used to characterize the global transcriptional responses of HOG1 and hog1 cells to three stress conditions that activate the Hog1 SAPK: osmotic stress, oxidative stress, and heavy metal stress. This revealed both stress-specific transcriptional responses and a core transcriptional response to stress in C. albicans. The core transcriptional response was characterized by a subset of genes that responded in a stereotypical manner to all of the stresses analyzed. Inactivation of HOG1 significantly attenuated transcriptional responses to osmotic and heavy metal stresses, but not to oxidative stress, and this was reflected in the role of Hog1 in the regulation of C. albicans core stress genes. Instead, the Capl transcription factor plays a key role in the oxidative stress regulation of C. albicans core stress genes. Our data show that the SAPK network in C. albicans has diverged from corresponding networks in model yeasts and that the C. albicans SAPK pathway functions in parallel with other pathways to regulate the core transcriptional response to stress.

Original languageEnglish
Pages (from-to)1018-1032
Number of pages14
JournalMolecular Biology of the Cell
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 2006

Keywords

  • OXIDATIVE STRESS
  • SACCHAROMYCES-CEREVISIAE
  • FISSION YEAST
  • OSMOTIC-STRESS
  • MAP KINASE
  • SCHIZOSACCHAROMYCES-POMBE
  • ENVIRONMENTAL-CHANGES
  • GENOME EXPRESSION
  • GENE DISRUPTION
  • FAMILY

Cite this

Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans.. / Enjalbert, Brice Patrick; Smith, D. A.; Cornell, M. J.; Alam, I.; Nicholls, Susan Melanie; Brown, Alistair James Petersen; Quinn, J.

In: Molecular Biology of the Cell, Vol. 17, No. 2, 02.2006, p. 1018-1032.

Research output: Contribution to journalArticle

Enjalbert, Brice Patrick ; Smith, D. A. ; Cornell, M. J. ; Alam, I. ; Nicholls, Susan Melanie ; Brown, Alistair James Petersen ; Quinn, J. / Role of the Hog1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 2. pp. 1018-1032.
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