Role of the N-terminal domain of phi C31 integrase in attB-attP synapsis

Paul A. Rowley, Margaret C. M. Smith

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

phi C31 integrase is a serine recombinase containing an N-terminal domain (NTD) that provides catalytic activity and a large C-terminal domain that controls which pair of DNA substrates is able to synapse. We show here that substitutions in amino acid V129 in the NTD can lead to defects in synapsis and DNA cleavage, indicating that the NTD also has an important role in synapsis.

Original languageEnglish
Pages (from-to)6918-6921
Number of pages4
JournalJournal of Bacteriology
Volume190
Issue number20
Early online date8 Aug 2008
DOIs
Publication statusPublished - Oct 2008

Keywords

  • site-specific recombination
  • BXB1 integration
  • DNA cleavage
  • resolvase
  • directionality

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