Root border cells take up and release glucose-C

V E C Stubbs, D Standing, O G G Knox, K Killham, A G Bengough, B Griffiths

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background and Aims Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.

Methods Border cells were removed from Zea mays L. root tips, and immersed in C-14-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.

Key Results Glucose uptake by the border cells increased with increasing glucose concentration from 0.2 to 20 mM. At 0.2 mM glucose, uptake was mainly active, as evidenced by the approx. 60% inhibition with phlorizin. At 2 and 20 mm glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mm glucose. There was no clear efflux/ time pattern for heat-killed border cells.

Conclusions Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions. (C) 2004 Annals of Botany Company.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalAnnals of Botany
Volume93
DOIs
Publication statusPublished - 2004

Keywords

  • glucose exchange
  • carbon flow
  • border cells
  • glucose uptake
  • sugar efflux
  • TORTUOSUS SUSPENSION CELLS
  • CAP CELLS
  • SUGAR CONCENTRATION
  • TRANSPORT
  • ZEA
  • PEA
  • RHIZOSPHERE
  • SUCROSE
  • PLASMALEMMA
  • PROTOPLASTS

Cite this

Stubbs, V. E. C., Standing, D., Knox, O. G. G., Killham, K., Bengough, A. G., & Griffiths, B. (2004). Root border cells take up and release glucose-C. Annals of Botany, 93, 221-224. https://doi.org/10.1093/aob/mch019

Root border cells take up and release glucose-C. / Stubbs, V E C ; Standing, D ; Knox, O G G ; Killham, K ; Bengough, A G ; Griffiths, B .

In: Annals of Botany, Vol. 93, 2004, p. 221-224.

Research output: Contribution to journalArticle

Stubbs, VEC, Standing, D, Knox, OGG, Killham, K, Bengough, AG & Griffiths, B 2004, 'Root border cells take up and release glucose-C', Annals of Botany, vol. 93, pp. 221-224. https://doi.org/10.1093/aob/mch019
Stubbs VEC, Standing D, Knox OGG, Killham K, Bengough AG, Griffiths B. Root border cells take up and release glucose-C. Annals of Botany. 2004;93:221-224. https://doi.org/10.1093/aob/mch019
Stubbs, V E C ; Standing, D ; Knox, O G G ; Killham, K ; Bengough, A G ; Griffiths, B . / Root border cells take up and release glucose-C. In: Annals of Botany. 2004 ; Vol. 93. pp. 221-224.
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abstract = "Background and Aims Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.Methods Border cells were removed from Zea mays L. root tips, and immersed in C-14-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.Key Results Glucose uptake by the border cells increased with increasing glucose concentration from 0.2 to 20 mM. At 0.2 mM glucose, uptake was mainly active, as evidenced by the approx. 60{\%} inhibition with phlorizin. At 2 and 20 mm glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mm glucose. There was no clear efflux/ time pattern for heat-killed border cells.Conclusions Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions. (C) 2004 Annals of Botany Company.",
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AU - Bengough, A G

AU - Griffiths, B

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N2 - Background and Aims Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.Methods Border cells were removed from Zea mays L. root tips, and immersed in C-14-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.Key Results Glucose uptake by the border cells increased with increasing glucose concentration from 0.2 to 20 mM. At 0.2 mM glucose, uptake was mainly active, as evidenced by the approx. 60% inhibition with phlorizin. At 2 and 20 mm glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mm glucose. There was no clear efflux/ time pattern for heat-killed border cells.Conclusions Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions. (C) 2004 Annals of Botany Company.

AB - Background and Aims Border cells are released from the root tips of many plant species, and can remain viable in the rhizosphere for 1 week. Whether border cells are capable of controlled glucose exchange with their environment was investigated.Methods Border cells were removed from Zea mays L. root tips, and immersed in C-14-labelled D-glucose. In one experiment, the hexose transport inhibitor, phlorizin, was used to investigate active glucose uptake from a range of glucose concentrations. In another experiment, glucose efflux from border cells was monitored over time.Key Results Glucose uptake by the border cells increased with increasing glucose concentration from 0.2 to 20 mM. At 0.2 mM glucose, uptake was mainly active, as evidenced by the approx. 60% inhibition with phlorizin. At 2 and 20 mm glucose, however, uptake was mainly via diffusion, as phlorizin inhibition was negligible. Glucose efflux increased with time for live border cells in both 2 and 20 mm glucose. There was no clear efflux/ time pattern for heat-killed border cells.Conclusions Border cells actively take up glucose, and also release it. Under our experimental conditions, glucose uptake and efflux were of similar order of magnitude. In the rhizosphere net glucose exchange will almost certainly depend on local soil conditions. (C) 2004 Annals of Botany Company.

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KW - SUGAR CONCENTRATION

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KW - ZEA

KW - PEA

KW - RHIZOSPHERE

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