Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes

A A Sneddon, P T Cohen, M J Stark

    Research output: Contribution to journalArticle

    130 Citations (Scopus)

    Abstract

    Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe. The PPH genes are genetically linked on chromosome IV and are predicted to encode polypeptides each with 74% amino acid sequence identity to rabbit type 2A protein phosphatase, indicating once again the extraordinarily high degree of sequence conservation shown by protein-phosphatases from different species. The two PPH genes show less than 10% amino acid sequence divergence from each other and while disruption of either PPH gene alone is without any major effect, the double disruption is lethal. This indicates that protein phosphatase 2A activity is an essential cellular function in yeast. Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell.
    Original languageEnglish
    Pages (from-to)4339-46
    Number of pages8
    JournalEMBO Journal
    Volume9
    Issue number13
    Publication statusPublished - 1990

    Fingerprint

    Saccharomyces cerevisiae Proteins
    Protein Phosphatase 2
    Genes
    Yeast
    Phosphoprotein Phosphatases
    Amino Acid Sequence
    Yeasts
    Rabbits
    Amino Acids
    Fungal Proteins
    Genomic Library
    Chromosomes
    Saccharomyces cerevisiae
    Conservation
    Complementary DNA
    Peptides

    Keywords

    • Alleles
    • Amino Acid Sequence
    • Animals
    • Base Sequence
    • DNA, Fungal
    • Genes, Fungal
    • Molecular Sequence Data
    • Phosphoprotein Phosphatases
    • Protein Phosphatase 2
    • Restriction Mapping
    • Saccharomyces cerevisiae
    • Xenopus

    Cite this

    Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. / Sneddon, A A; Cohen, P T; Stark, M J.

    In: EMBO Journal, Vol. 9, No. 13, 1990, p. 4339-46.

    Research output: Contribution to journalArticle

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    abstract = "Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe. The PPH genes are genetically linked on chromosome IV and are predicted to encode polypeptides each with 74{\%} amino acid sequence identity to rabbit type 2A protein phosphatase, indicating once again the extraordinarily high degree of sequence conservation shown by protein-phosphatases from different species. The two PPH genes show less than 10{\%} amino acid sequence divergence from each other and while disruption of either PPH gene alone is without any major effect, the double disruption is lethal. This indicates that protein phosphatase 2A activity is an essential cellular function in yeast. Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell.",
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    T1 - Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes

    AU - Sneddon, A A

    AU - Cohen, P T

    AU - Stark, M J

    PY - 1990

    Y1 - 1990

    N2 - Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe. The PPH genes are genetically linked on chromosome IV and are predicted to encode polypeptides each with 74% amino acid sequence identity to rabbit type 2A protein phosphatase, indicating once again the extraordinarily high degree of sequence conservation shown by protein-phosphatases from different species. The two PPH genes show less than 10% amino acid sequence divergence from each other and while disruption of either PPH gene alone is without any major effect, the double disruption is lethal. This indicates that protein phosphatase 2A activity is an essential cellular function in yeast. Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell.

    AB - Two genes (PPH21 and PPH22) encoding the yeast homologues of protein serine-threonine phosphatase 2A have been cloned from a Saccharomyces cerevisiae genomic library using a rabbit protein phosphatase 2A cDNA as a hybridization probe. The PPH genes are genetically linked on chromosome IV and are predicted to encode polypeptides each with 74% amino acid sequence identity to rabbit type 2A protein phosphatase, indicating once again the extraordinarily high degree of sequence conservation shown by protein-phosphatases from different species. The two PPH genes show less than 10% amino acid sequence divergence from each other and while disruption of either PPH gene alone is without any major effect, the double disruption is lethal. This indicates that protein phosphatase 2A activity is an essential cellular function in yeast. Measurement of type 2A protein phosphatase activity in yeast strains lacking one or other of the genes indicates that they account for most, if not all, protein phosphatase 2A activity in the cell.

    KW - Alleles

    KW - Amino Acid Sequence

    KW - Animals

    KW - Base Sequence

    KW - DNA, Fungal

    KW - Genes, Fungal

    KW - Molecular Sequence Data

    KW - Phosphoprotein Phosphatases

    KW - Protein Phosphatase 2

    KW - Restriction Mapping

    KW - Saccharomyces cerevisiae

    KW - Xenopus

    M3 - Article

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    VL - 9

    SP - 4339

    EP - 4346

    JO - EMBO Journal

    JF - EMBO Journal

    SN - 0261-4189

    IS - 13

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