Sandeels in the diets of seals

application of novel and conventional methods of analysis to faeces from seals in the Moray Firth area of Scotland

Graham John Pierce, Peter Robin Boyle, Jane Diack, I Clark

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Serological methods for prey identification have been applied to detection of residues of sandeel (Ammodytidae) protein in faeces of common seals (Phoca vitulina) and grey seals (Halichoerus grypus) from the Moray Firth, north-east Scotland. Antisera raised to muscle protein from Ammodytes marinus were evaluated by testing their reactions with protein extracts made from a range of North Sea fish species and protein residues in in vitro digestates, seal digestive tracts and seal faeces. It was concluded that, using fused rocket immuno-electrophoresis, linkage of precipitin peaks from unknown samples with peaks from standard sandeel extract was a reliable indicator of the presence of sandeel in the unknown sample. Seasonal variation in the incidence of sandeels in common seal diet in the Moray Firth was examined by identifying otoliths, bones, and proteins, and all three methods indicated that sandeels occurred in the majority of samples tested in the summer, but were less important during the winter. Proteins were detected in fewer samples than otoliths, particularly in February and March. Possible reasons for this difference are discussed. Serological identification of sandeel proteins is potentially applicable to dietary studies on all marine predators.
Original languageEnglish
Pages (from-to)829-840
Number of pages12
JournalJournal of the Marine Biological Association of the United Kingdom
Volume70
Issue number4
DOIs
Publication statusPublished - Nov 1990

Cite this

Sandeels in the diets of seals : application of novel and conventional methods of analysis to faeces from seals in the Moray Firth area of Scotland. / Pierce, Graham John; Boyle, Peter Robin; Diack, Jane; Clark, I .

In: Journal of the Marine Biological Association of the United Kingdom, Vol. 70, No. 4, 11.1990, p. 829-840.

Research output: Contribution to journalArticle

@article{89bca506c2094e59a4a9e5a08d2d8649,
title = "Sandeels in the diets of seals: application of novel and conventional methods of analysis to faeces from seals in the Moray Firth area of Scotland",
abstract = "Serological methods for prey identification have been applied to detection of residues of sandeel (Ammodytidae) protein in faeces of common seals (Phoca vitulina) and grey seals (Halichoerus grypus) from the Moray Firth, north-east Scotland. Antisera raised to muscle protein from Ammodytes marinus were evaluated by testing their reactions with protein extracts made from a range of North Sea fish species and protein residues in in vitro digestates, seal digestive tracts and seal faeces. It was concluded that, using fused rocket immuno-electrophoresis, linkage of precipitin peaks from unknown samples with peaks from standard sandeel extract was a reliable indicator of the presence of sandeel in the unknown sample. Seasonal variation in the incidence of sandeels in common seal diet in the Moray Firth was examined by identifying otoliths, bones, and proteins, and all three methods indicated that sandeels occurred in the majority of samples tested in the summer, but were less important during the winter. Proteins were detected in fewer samples than otoliths, particularly in February and March. Possible reasons for this difference are discussed. Serological identification of sandeel proteins is potentially applicable to dietary studies on all marine predators.",
author = "Pierce, {Graham John} and Boyle, {Peter Robin} and Jane Diack and I Clark",
year = "1990",
month = "11",
doi = "10.1017/S0025315400059099",
language = "English",
volume = "70",
pages = "829--840",
journal = "Journal of the Marine Biological Association of the United Kingdom",
issn = "0025-3154",
publisher = "Cambridge University Press",
number = "4",

}

TY - JOUR

T1 - Sandeels in the diets of seals

T2 - application of novel and conventional methods of analysis to faeces from seals in the Moray Firth area of Scotland

AU - Pierce, Graham John

AU - Boyle, Peter Robin

AU - Diack, Jane

AU - Clark, I

PY - 1990/11

Y1 - 1990/11

N2 - Serological methods for prey identification have been applied to detection of residues of sandeel (Ammodytidae) protein in faeces of common seals (Phoca vitulina) and grey seals (Halichoerus grypus) from the Moray Firth, north-east Scotland. Antisera raised to muscle protein from Ammodytes marinus were evaluated by testing their reactions with protein extracts made from a range of North Sea fish species and protein residues in in vitro digestates, seal digestive tracts and seal faeces. It was concluded that, using fused rocket immuno-electrophoresis, linkage of precipitin peaks from unknown samples with peaks from standard sandeel extract was a reliable indicator of the presence of sandeel in the unknown sample. Seasonal variation in the incidence of sandeels in common seal diet in the Moray Firth was examined by identifying otoliths, bones, and proteins, and all three methods indicated that sandeels occurred in the majority of samples tested in the summer, but were less important during the winter. Proteins were detected in fewer samples than otoliths, particularly in February and March. Possible reasons for this difference are discussed. Serological identification of sandeel proteins is potentially applicable to dietary studies on all marine predators.

AB - Serological methods for prey identification have been applied to detection of residues of sandeel (Ammodytidae) protein in faeces of common seals (Phoca vitulina) and grey seals (Halichoerus grypus) from the Moray Firth, north-east Scotland. Antisera raised to muscle protein from Ammodytes marinus were evaluated by testing their reactions with protein extracts made from a range of North Sea fish species and protein residues in in vitro digestates, seal digestive tracts and seal faeces. It was concluded that, using fused rocket immuno-electrophoresis, linkage of precipitin peaks from unknown samples with peaks from standard sandeel extract was a reliable indicator of the presence of sandeel in the unknown sample. Seasonal variation in the incidence of sandeels in common seal diet in the Moray Firth was examined by identifying otoliths, bones, and proteins, and all three methods indicated that sandeels occurred in the majority of samples tested in the summer, but were less important during the winter. Proteins were detected in fewer samples than otoliths, particularly in February and March. Possible reasons for this difference are discussed. Serological identification of sandeel proteins is potentially applicable to dietary studies on all marine predators.

U2 - 10.1017/S0025315400059099

DO - 10.1017/S0025315400059099

M3 - Article

VL - 70

SP - 829

EP - 840

JO - Journal of the Marine Biological Association of the United Kingdom

JF - Journal of the Marine Biological Association of the United Kingdom

SN - 0025-3154

IS - 4

ER -