Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1

Madhusudhan Srinivasan* (Corresponding Author), Naomi J Petela, Johanna C Scheinost, James Collier, Menelaos Voulgaris, Maurici B Roig, Frederic Beckouët, Bin Hu, Kim A Nasmyth*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)
1 Downloads (Pure)

Abstract

Cohesin’s association with chromosomes is determined by loading dependent on the Scc2/4 complex and release due to Wapl. We show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1’s ATPase. The Wapl-independent release mechanism is switched off as cells activate Cdk1 and enter G2/M and cannot be turned back on without cohesin’s dissociation from chromosomes. The latter phenomenon enabled us to show that in the absence of release mechanisms, cohesin rings that have already captured DNA in a Scc2-dependent manner before replication no longer require Scc2 to capture sister DNAs during S phase.
Original languageEnglish
Article numbere44736
Number of pages34
JournaleLife
Volume8
Early online date21 Jun 2019
DOIs
Publication statusPublished - 21 Jun 2019

Fingerprint Dive into the research topics of 'Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1'. Together they form a unique fingerprint.

  • Cite this

    Srinivasan, M., Petela, N. J., Scheinost, J. C., Collier, J., Voulgaris, M., Roig, M. B., Beckouët, F., Hu, B., & Nasmyth, K. A. (2019). Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1. eLife, 8, [e44736]. https://doi.org/10.7554/elife.44736