Sciatic nerve of diabetic rat treated with epoetin delta: effects on C-Fibers and blood vessels including pericytes

Andrzej Loesch, Helen Tang, Mary A Cotter, Norman E Cameron

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In diabetes mellitus (DM) reduced motor and sensory properties of peripheral nerves are linked with the dysfunction of neural vasculature. We investigated C-fibers and microvessels of sciatic nerve of normal, DM, and DM + epoetin delta-treated rats. C-fibers immunoreactive for calcitonin gene-related peptide (CGRP), tyrosine hydroxylase (TH), epoetin receptor (EpoR), and common ß receptor subunit of the interleukin 3 receptor (IL-3Rß) were present in all rats, whereas in DM and epoetin-treated rats C-fibers also showed neuronal (nNOS) and inducible (iNOS) nitric oxide synthases. The cross-sectional area of CGRPpositive C-fibers was decreased in DM, but it recovered after epoetin treatment. In all conditions, vascular endothelium showed scarce immunolabeling for endothelial nitric oxide synthase (eNOS); the profound immunoreactivity for eNOS, EpoR, and IL-3Rß was in pericytes. Some perivascular autonomic nerves were damaged and IL-3Rß positive. Findings are discussed in terms of declined sensory conduction velocity in DM, its improvement after epoetin treatment, and the possible vascular contribution to these phenomena.
Original languageEnglish
Pages (from-to)651-668
Number of pages18
JournalAngiology
Volume61
Issue number7
Early online date13 Jun 2010
DOIs
Publication statusPublished - Oct 2010

Keywords

  • epoetin
  • diabetes
  • sciatic nerve
  • electron immunocytochemistry
  • rat

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