Abstract
SEARCHBreast, a UK initiative supported by the NC3Rs, organised a workshop entitled 3D Modelling of Breast Cancer. The workshop focused on providing researchers with solutions to overcome some of the perceived barriers to working with human-derived tumour cells, cell lines and tissues, namely: a) the limited access to human-derived material; and b) the difficulty in working with these samples. The workshop presentations provided constructive advice and information on how to best prepare human cells or tissues for further downstream applications. Techniques in developing primary cultures from patient samples, and considerations when preserving tissue slices, were discussed. A common theme throughout the workshop was the importance of ensuring that the cells are grown in conditions as similar to the in vivo microenvironment as possible. Comparisons of the advantages of several in vitro options, such as primary cell cultures, cell line cultures, explants or tissue slices, suggest that all offer great potential applications for breast cancer research, and highlight that it need not be a case of choosing one over the other. The workshop also offered cutting-edge examples of on-chip technologies and 3-D tumour modelling by using virtual pathology, which can contribute to clinically relevant studies and provide insights into breast cancer metastatic mechanisms.
| Original language | English |
|---|---|
| Pages (from-to) | 367-375 |
| Number of pages | 9 |
| Journal | ATLA Alternatives to Laboratory Animals |
| Volume | 43 |
| Issue number | 6 |
| Publication status | Published - 1 Dec 2015 |
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Keywords
- 3-D models
- Animal replacement
- Breast cancer
- Humanised breast cancer models
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Toxicology
- Medical Laboratory Technology
Cite this
SEARCHBreast workshop proceedings : 3D modelling of breast cancer. / Morrissey, Bethny; Blyth, Karen; Carter, Phil; Chelala, Claude; Holen, Ingunn; Jones, Louise; Speirs, Valerie.
In: ATLA Alternatives to Laboratory Animals, Vol. 43, No. 6, 01.12.2015, p. 367-375.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - SEARCHBreast workshop proceedings
T2 - 3D modelling of breast cancer
AU - Morrissey, Bethny
AU - Blyth, Karen
AU - Carter, Phil
AU - Chelala, Claude
AU - Holen, Ingunn
AU - Jones, Louise
AU - Speirs, Valerie
PY - 2015/12/1
Y1 - 2015/12/1
N2 - SEARCHBreast, a UK initiative supported by the NC3Rs, organised a workshop entitled 3D Modelling of Breast Cancer. The workshop focused on providing researchers with solutions to overcome some of the perceived barriers to working with human-derived tumour cells, cell lines and tissues, namely: a) the limited access to human-derived material; and b) the difficulty in working with these samples. The workshop presentations provided constructive advice and information on how to best prepare human cells or tissues for further downstream applications. Techniques in developing primary cultures from patient samples, and considerations when preserving tissue slices, were discussed. A common theme throughout the workshop was the importance of ensuring that the cells are grown in conditions as similar to the in vivo microenvironment as possible. Comparisons of the advantages of several in vitro options, such as primary cell cultures, cell line cultures, explants or tissue slices, suggest that all offer great potential applications for breast cancer research, and highlight that it need not be a case of choosing one over the other. The workshop also offered cutting-edge examples of on-chip technologies and 3-D tumour modelling by using virtual pathology, which can contribute to clinically relevant studies and provide insights into breast cancer metastatic mechanisms.
AB - SEARCHBreast, a UK initiative supported by the NC3Rs, organised a workshop entitled 3D Modelling of Breast Cancer. The workshop focused on providing researchers with solutions to overcome some of the perceived barriers to working with human-derived tumour cells, cell lines and tissues, namely: a) the limited access to human-derived material; and b) the difficulty in working with these samples. The workshop presentations provided constructive advice and information on how to best prepare human cells or tissues for further downstream applications. Techniques in developing primary cultures from patient samples, and considerations when preserving tissue slices, were discussed. A common theme throughout the workshop was the importance of ensuring that the cells are grown in conditions as similar to the in vivo microenvironment as possible. Comparisons of the advantages of several in vitro options, such as primary cell cultures, cell line cultures, explants or tissue slices, suggest that all offer great potential applications for breast cancer research, and highlight that it need not be a case of choosing one over the other. The workshop also offered cutting-edge examples of on-chip technologies and 3-D tumour modelling by using virtual pathology, which can contribute to clinically relevant studies and provide insights into breast cancer metastatic mechanisms.
KW - 3-D models
KW - Animal replacement
KW - Breast cancer
KW - Humanised breast cancer models
UR - http://www.scopus.com/inward/record.url?scp=84956939025&partnerID=8YFLogxK
M3 - Article
C2 - 26753939
AN - SCOPUS:84956939025
VL - 43
SP - 367
EP - 375
JO - ATLA-Alternatives to Laboratory Animals
JF - ATLA-Alternatives to Laboratory Animals
SN - 0261-1929
IS - 6
ER -