Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae

S Kuratli, A Hemphill, J Lindh, D F Smith, Bernadette Connolly

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Trichinella tsJ5 gene is preferentially expressed in muscle larvae of Trichinella spiralis and encodes a novel protein. Previous observations have shown tsJ5 to be expressed at higher levels in encapsulating species than in non-encapsulating species and down-regulation of gene expression in T. pseudospiralis to be correlated with a lower protein abundance in the muscle larva of this species. In the present study we have determined the full-length cDNA sequence of the tsJ5 homologue in T. pseudospiralis (tpJ5). Antigens recognised by an anti-J5 antibody are found on the cuticular surface of both T. spiralis and T. pseudospiralis muscle larvae, as well as in the body wall muscle. We show that both the TSJ5 and TPJ5 proteins are found in the excretory/secretory fractions collected from muscle larva cultured in vitro and that despite the absence of a typical N-terminal signal sequence, secretion of pTSJ5 is mediated through the classical ER/Golgi secretory pathway.
Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalMolecular and Biochemical Parasitology
Volume115
Issue number2
DOIs
Publication statusPublished - 1 Jul 2001

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Trichinella
Larva
Muscles
Trichinella spiralis
Proteins
Secretory Pathway
Gene Expression Regulation
Sequence Homology
Protein Sorting Signals
Anti-Idiotypic Antibodies
Down-Regulation
Complementary DNA
Antigens
Genes

Keywords

  • Amino Acid Sequence
  • Animals
  • DNA, Complementary
  • DNA, Helminth
  • Fluorescent Antibody Technique
  • Helminth Proteins
  • Larva
  • Mice
  • Mice, Inbred ICR
  • Molecular Sequence Data
  • Muscle Proteins
  • Muscle, Skeletal
  • Repressor Proteins
  • Sequence Analysis, DNA
  • Trichinella
  • Trichinella spiralis
  • Trichinellosis
  • Nematode
  • Secretion
  • Immuno-localisation
  • Crude worm extract
  • Excretory-secretory
  • ER/Golgi

Cite this

Secretion of the novel Trichinella protein TSJ5 by T. spiralis and T. pseudospiralis muscle larvae. / Kuratli, S; Hemphill, A; Lindh, J; Smith, D F; Connolly, Bernadette.

In: Molecular and Biochemical Parasitology, Vol. 115, No. 2, 01.07.2001, p. 199-208.

Research output: Contribution to journalArticle

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