Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments

Alvar Trucco, Roman S Polishchuk, Oliviano Martella, Alessio Di Pentima, Aurora Fusella, Daniele Di Giandomenico, Enrica San Pietro, Galina V Beznoussenko, Elena V Polishchuk, Massimiliano Baldassarre, Roberto Buccione, Willie J C Geerts, Abraham J Koster, Koert N J Burger, Alexander A Mironov, Alberto Luini

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The organization of secretory traffic remains unclear, mainly because of the complex structure and dynamics of the secretory pathway. We have thus studied a simplified system, a single synchronized traffic wave crossing an individual Golgi stack, using electron tomography. Endoplasmic-reticulum-to-Golgi carriers join the stack by fusing with cis cisternae and induce the formation of intercisternal tubules, through which they redistribute their contents throughout the stack. These tubules seem to be pervious to Golgi enzymes, whereas Golgi vesicles are depleted of both enzymes and cargo. Cargo then traverses the stack without leaving the cisternal lumen. When cargo exits the stack, intercisternal connections disappear. These findings provide a new view of secretory traffic that includes dynamic intercompartment continuities as key players.

Original languageEnglish
Pages (from-to)1071-81
Number of pages11
JournalNature Cell Biology
Volume6
Issue number11
DOIs
Publication statusPublished - Nov 2004

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Electron Microscope Tomography
Secretory Pathway
Enzymes
Endoplasmic Reticulum

Keywords

  • Biological Transport
  • Cell Compartmentation
  • Cell Line
  • Endoplasmic Reticulum
  • Golgi Apparatus
  • Microscopy, Electron

Cite this

Trucco, A., Polishchuk, R. S., Martella, O., Di Pentima, A., Fusella, A., Di Giandomenico, D., ... Luini, A. (2004). Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments. Nature Cell Biology, 6(11), 1071-81. https://doi.org/10.1038/ncb1180

Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments. / Trucco, Alvar; Polishchuk, Roman S; Martella, Oliviano; Di Pentima, Alessio; Fusella, Aurora; Di Giandomenico, Daniele; San Pietro, Enrica; Beznoussenko, Galina V; Polishchuk, Elena V; Baldassarre, Massimiliano; Buccione, Roberto; Geerts, Willie J C; Koster, Abraham J; Burger, Koert N J; Mironov, Alexander A; Luini, Alberto.

In: Nature Cell Biology, Vol. 6, No. 11, 11.2004, p. 1071-81.

Research output: Contribution to journalArticle

Trucco, A, Polishchuk, RS, Martella, O, Di Pentima, A, Fusella, A, Di Giandomenico, D, San Pietro, E, Beznoussenko, GV, Polishchuk, EV, Baldassarre, M, Buccione, R, Geerts, WJC, Koster, AJ, Burger, KNJ, Mironov, AA & Luini, A 2004, 'Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments', Nature Cell Biology, vol. 6, no. 11, pp. 1071-81. https://doi.org/10.1038/ncb1180
Trucco A, Polishchuk RS, Martella O, Di Pentima A, Fusella A, Di Giandomenico D et al. Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments. Nature Cell Biology. 2004 Nov;6(11):1071-81. https://doi.org/10.1038/ncb1180
Trucco, Alvar ; Polishchuk, Roman S ; Martella, Oliviano ; Di Pentima, Alessio ; Fusella, Aurora ; Di Giandomenico, Daniele ; San Pietro, Enrica ; Beznoussenko, Galina V ; Polishchuk, Elena V ; Baldassarre, Massimiliano ; Buccione, Roberto ; Geerts, Willie J C ; Koster, Abraham J ; Burger, Koert N J ; Mironov, Alexander A ; Luini, Alberto. / Secretory traffic triggers the formation of tubular continuities across Golgi sub-compartments. In: Nature Cell Biology. 2004 ; Vol. 6, No. 11. pp. 1071-81.
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