Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption

John Hamilton Beattie, Martin David Reid, L J Harvey, J R Dainty, G Majsak-Newman, S J Fairweather-Tait

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Measuring mineral absorption by fecal monitoring is labor-intensive and relies on good volunteer compliance. Blood indicators of absorption could be advantageous and we have developed a method for selective extraction of recently absorbed (exchangeable) copper based on dialysis of plasma with histidine and subsequent copper extraction using Chelex resin. The potential for measuring copper absorption by transient enrichment of exchangeable copper with the stable isotope Cu-65 from an ingested tracer, was also investigated. This method was compared with that of the fecal monitoring technique in a human volunteer, who consumed a 6 mg dose of Cu-65 with inhibitors of copper absorption. Holmium was used as a non-absorbable rare-earth marker of unabsorbed tracer excretion, allowing estimation of re-secreted Cu-65 (44 mug d(-1)), and hence calculation of true tracer absorption, which was only 10.8%. Monitoring plasma tracer kinetics showed potential for estimation of copper absorption without the need for fecal copper analysis.

    Original languageEnglish
    Pages (from-to)2225-2229
    Number of pages5
    JournalAnalyst
    Volume126
    Issue number12
    Publication statusPublished - 2001

    Keywords

    • determine bioavailability
    • stable isotopes
    • ceruloplasmin
    • metabolism
    • elements
    • adult
    • men

    Cite this

    Beattie, J. H., Reid, M. D., Harvey, L. J., Dainty, J. R., Majsak-Newman, G., & Fairweather-Tait, S. J. (2001). Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption. Analyst, 126(12), 2225-2229.

    Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption. / Beattie, John Hamilton; Reid, Martin David; Harvey, L J ; Dainty, J R ; Majsak-Newman, G ; Fairweather-Tait, S J .

    In: Analyst, Vol. 126, No. 12, 2001, p. 2225-2229.

    Research output: Contribution to journalArticle

    Beattie, JH, Reid, MD, Harvey, LJ, Dainty, JR, Majsak-Newman, G & Fairweather-Tait, SJ 2001, 'Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption', Analyst, vol. 126, no. 12, pp. 2225-2229.
    Beattie JH, Reid MD, Harvey LJ, Dainty JR, Majsak-Newman G, Fairweather-Tait SJ. Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption. Analyst. 2001;126(12):2225-2229.
    Beattie, John Hamilton ; Reid, Martin David ; Harvey, L J ; Dainty, J R ; Majsak-Newman, G ; Fairweather-Tait, S J . / Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption. In: Analyst. 2001 ; Vol. 126, No. 12. pp. 2225-2229.
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    AU - Reid, Martin David

    AU - Harvey, L J

    AU - Dainty, J R

    AU - Majsak-Newman, G

    AU - Fairweather-Tait, S J

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    KW - metabolism

    KW - elements

    KW - adult

    KW - men

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