Selenite and selenate affected the fatty acid profile in in vitro incubated ovine ruminal fluid containing linoleic acid

M. Czauderna, J. Kowalczyk, R. J. Wallace

Research output: Contribution to journalArticle

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Abstract

The influence of adding selenite (Se-IV) or selenate (Se-VI) to ovine ruminal fluid containing linoleic acid (LA) on the profile of fatty acids, especially conjugated linoleic acid (CLA) isomers and their metabolites was investigated. Dietary LA is incorporated by rumen bacteria, isomerized to other geometric and positional isomers, metabolized into CLA isomers, biohydrogenated to trans-vaccenic acid (TVA) and finally to C18:0. Considering the above, ovine ruminal fluid was incubated in vitro at 39 degrees C under CO2 either alone (the control ruminal fluid) or with a combination of LA (1.67 mg/ml), a low (0.167 mu g/ml) or high (1.67 mu g/ml) level of selenium as Se-IV or Se-VI. Tubes with examined ruminal fluid were removed after 0, 6, 12, 18, and 24 hrs of incubation and then submitted for determination of fatty acids (FA). FA, as methyl esters, were quantitated using capillary gas chromatography and flame-ionization detection. Both concentrations of Se-IV added to the ruminal fluid with LA usually decreased the concentrations of individual CLA isomers, especially cis9trans11CLA (c9t11CLA) and the sum of all CLA isomers in the ruminal fluid in comparison with the fluid containing only LA. Our studies documented that Se-IV reduced the capacity of bacterial isomerase, which turns the cis9-bond into a trans10-bond. The addition of Se-IV to the ruminal fluid with LA decreased the concentration of TVA compared with the fluid with only LA; a decrease in the loss of TVA was observed with increasing concentrations of Se-IV. The presence of Se-IV in the ovine fluid with LA stimulated the biohydrogenation of TVA to C18:0. The addition of LA to the incubated fluid, irrespectively of the presence of Se-IV, increased the concentration of C20:5n-3. Se-VI in the ruminal fluid with LA usually more efficiently increased the concentration of c9t11CLA, t10c12CLA, c9c11CLA and t9t11CLA, from 6 until 24 hrs of incubation compared with the fluid containing LA, regardless of the presence of Se-IV. The concentration of TVA in the fluid containing Se-VI and LA is higher than in the fluid with Se-IV and LA. Se-VI in the fluid increased the concentration of C18:0. As a consequence, Se-VI added to the fluid increased the yield of final biohydrogenation to C18:0 compared with the fluid with LA, irrespective of the presence of Se-VI. Further studies are required to clarify the effects of other Se-compounds and fatty acids on concentrations of fatty acids, especially CLA isomers and their precursors, in the ruminal fluid
Original languageEnglish
Pages (from-to)477-492
Number of pages16
JournalJournal of Animal and Feed Sciences
Volume21
Issue number3
Publication statusPublished - 2012

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Selenic Acid
Selenious Acid
selenates
selenites
Linoleic Acid
rumen fluids
linoleic acid
Sheep
Fatty Acids
fatty acid composition
sheep
Conjugated Linoleic Acids
vaccenic acid
isomers
conjugated linoleic acid
fatty acids
biohydrogenation
In Vitro Techniques
fluids
Flame Ionization

Keywords

  • ruminal fluid
  • CLA isomers
  • fatty acid
  • linoleic acid
  • selenium
  • sheep

Cite this

Selenite and selenate affected the fatty acid profile in in vitro incubated ovine ruminal fluid containing linoleic acid. / Czauderna, M.; Kowalczyk, J.; Wallace, R. J.

In: Journal of Animal and Feed Sciences, Vol. 21, No. 3, 2012, p. 477-492.

Research output: Contribution to journalArticle

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N1 - Times Cited: 0 KIELANOWSKI INST ANIMAL PHYSIOLOGY NUTRITION UL INSTYTUCKA 3, 05-110 JABLONNA, POLAND JABLONNA Article 018FA English Cited References Count: 24 Czauderna, M Polish Acad Sci, Kielanowski Inst Anim Physiol & Nutr, PL-05110 Jablonna, Poland

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N2 - The influence of adding selenite (Se-IV) or selenate (Se-VI) to ovine ruminal fluid containing linoleic acid (LA) on the profile of fatty acids, especially conjugated linoleic acid (CLA) isomers and their metabolites was investigated. Dietary LA is incorporated by rumen bacteria, isomerized to other geometric and positional isomers, metabolized into CLA isomers, biohydrogenated to trans-vaccenic acid (TVA) and finally to C18:0. Considering the above, ovine ruminal fluid was incubated in vitro at 39 degrees C under CO2 either alone (the control ruminal fluid) or with a combination of LA (1.67 mg/ml), a low (0.167 mu g/ml) or high (1.67 mu g/ml) level of selenium as Se-IV or Se-VI. Tubes with examined ruminal fluid were removed after 0, 6, 12, 18, and 24 hrs of incubation and then submitted for determination of fatty acids (FA). FA, as methyl esters, were quantitated using capillary gas chromatography and flame-ionization detection. Both concentrations of Se-IV added to the ruminal fluid with LA usually decreased the concentrations of individual CLA isomers, especially cis9trans11CLA (c9t11CLA) and the sum of all CLA isomers in the ruminal fluid in comparison with the fluid containing only LA. Our studies documented that Se-IV reduced the capacity of bacterial isomerase, which turns the cis9-bond into a trans10-bond. The addition of Se-IV to the ruminal fluid with LA decreased the concentration of TVA compared with the fluid with only LA; a decrease in the loss of TVA was observed with increasing concentrations of Se-IV. The presence of Se-IV in the ovine fluid with LA stimulated the biohydrogenation of TVA to C18:0. The addition of LA to the incubated fluid, irrespectively of the presence of Se-IV, increased the concentration of C20:5n-3. Se-VI in the ruminal fluid with LA usually more efficiently increased the concentration of c9t11CLA, t10c12CLA, c9c11CLA and t9t11CLA, from 6 until 24 hrs of incubation compared with the fluid containing LA, regardless of the presence of Se-IV. The concentration of TVA in the fluid containing Se-VI and LA is higher than in the fluid with Se-IV and LA. Se-VI in the fluid increased the concentration of C18:0. As a consequence, Se-VI added to the fluid increased the yield of final biohydrogenation to C18:0 compared with the fluid with LA, irrespective of the presence of Se-VI. Further studies are required to clarify the effects of other Se-compounds and fatty acids on concentrations of fatty acids, especially CLA isomers and their precursors, in the ruminal fluid

AB - The influence of adding selenite (Se-IV) or selenate (Se-VI) to ovine ruminal fluid containing linoleic acid (LA) on the profile of fatty acids, especially conjugated linoleic acid (CLA) isomers and their metabolites was investigated. Dietary LA is incorporated by rumen bacteria, isomerized to other geometric and positional isomers, metabolized into CLA isomers, biohydrogenated to trans-vaccenic acid (TVA) and finally to C18:0. Considering the above, ovine ruminal fluid was incubated in vitro at 39 degrees C under CO2 either alone (the control ruminal fluid) or with a combination of LA (1.67 mg/ml), a low (0.167 mu g/ml) or high (1.67 mu g/ml) level of selenium as Se-IV or Se-VI. Tubes with examined ruminal fluid were removed after 0, 6, 12, 18, and 24 hrs of incubation and then submitted for determination of fatty acids (FA). FA, as methyl esters, were quantitated using capillary gas chromatography and flame-ionization detection. Both concentrations of Se-IV added to the ruminal fluid with LA usually decreased the concentrations of individual CLA isomers, especially cis9trans11CLA (c9t11CLA) and the sum of all CLA isomers in the ruminal fluid in comparison with the fluid containing only LA. Our studies documented that Se-IV reduced the capacity of bacterial isomerase, which turns the cis9-bond into a trans10-bond. The addition of Se-IV to the ruminal fluid with LA decreased the concentration of TVA compared with the fluid with only LA; a decrease in the loss of TVA was observed with increasing concentrations of Se-IV. The presence of Se-IV in the ovine fluid with LA stimulated the biohydrogenation of TVA to C18:0. The addition of LA to the incubated fluid, irrespectively of the presence of Se-IV, increased the concentration of C20:5n-3. Se-VI in the ruminal fluid with LA usually more efficiently increased the concentration of c9t11CLA, t10c12CLA, c9c11CLA and t9t11CLA, from 6 until 24 hrs of incubation compared with the fluid containing LA, regardless of the presence of Se-IV. The concentration of TVA in the fluid containing Se-VI and LA is higher than in the fluid with Se-IV and LA. Se-VI in the fluid increased the concentration of C18:0. As a consequence, Se-VI added to the fluid increased the yield of final biohydrogenation to C18:0 compared with the fluid with LA, irrespective of the presence of Se-VI. Further studies are required to clarify the effects of other Se-compounds and fatty acids on concentrations of fatty acids, especially CLA isomers and their precursors, in the ruminal fluid

KW - ruminal fluid

KW - CLA isomers

KW - fatty acid

KW - linoleic acid

KW - selenium

KW - sheep

M3 - Article

VL - 21

SP - 477

EP - 492

JO - Journal of Animal and Feed Sciences

JF - Journal of Animal and Feed Sciences

SN - 1230-1388

IS - 3

ER -