Selenium Supplementation in Fish

A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss)

Davide Pacitti, Muhammad M. Lawan, John Sweetman, Samuel A.M. Martin, Jörg Feldmann, Christopher J. Secombes

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Abstract

Background

Selenium (Se) is an essential oligonutrient, as a component of several Se-containing proteins (selenoproteins), which exert important biological functions within an organism. In livestock, Se-enriched products have been proposed as dietary supplements to be included into functional feeds for animal preventive health care. To this end, it is important to understand the optimal range of concentrations for supplementation and how long it takes to be assimilated into the organism.

Methods

In this study, rainbow trout (Oncorhynchus mykiss) were fed a control diet containing 0.9 g Kg-1 Se or the same diet supplemented with a Se-Yeast product (Sel-Plex) to achieve Se concentrations ranging from 1.5–8.9 g Kg-1 for a period of ten weeks. Fish were sampled every two weeks for analysis. The kinetics of Se bioaccumulation and the effects on fish selenoprotein expression was determined in different tissues combining chemical and bimolecular techniques.

Results

The Sel-Plex enriched diets did not have any effect on survival and growth performance. The highest Se levels were found in liver and kidney followed by muscle and blood cells. Analysis of the Se concentration factor showed that liver is able to initially regulate the amount of Se accumulated. However, with higher dietary Se level (4.8 and 8.9 g Kg-1) and longer times of exposure (10 weeks), regulation is ineffective and the Se tissue concentration increases. The expression of the selected trout selenoprotein transcripts showed an inverse correlation with Sel-Plex augmentation in most cases. In liver, kidney and blood cells the highest up-regulation of the trout selenoprotein genes was seen mostly in the group fed the diet enriched with the lowest concentration of Sel-Plex (0.5 g Kg-1) for 10 weeks.

Conclusion

Sel-Plex may represent an excellent Se supplement to deliver a high level of Se without provoking harm to the fish and to guarantee the maximal absorption of the element. According to our results, a dietary supplementation of Sel-Plex between 0.5 and 4 g Kg-1 may allow maximal benefits, whereas 8 g Kg-1 may be excessive for the purpose of supplementation.
Original languageEnglish
Article numbere0127041
Number of pages17
JournalPloS ONE
Volume10
Issue number5
Early online date15 May 2015
DOIs
Publication statusPublished - 15 May 2015

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Selenoproteins
selenoproteins
Oncorhynchus mykiss
Nutrition
Selenium
Fish
selenium
assimilation (physiology)
Fishes
Liver
Diet
Trout
blood cells
Dietary Supplements
fish
diet
trout
dietary supplements
Blood Cells
Blood

Cite this

@article{6382a89e61a447c4969a52b83fa27168,
title = "Selenium Supplementation in Fish: A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss)",
abstract = "BackgroundSelenium (Se) is an essential oligonutrient, as a component of several Se-containing proteins (selenoproteins), which exert important biological functions within an organism. In livestock, Se-enriched products have been proposed as dietary supplements to be included into functional feeds for animal preventive health care. To this end, it is important to understand the optimal range of concentrations for supplementation and how long it takes to be assimilated into the organism.MethodsIn this study, rainbow trout (Oncorhynchus mykiss) were fed a control diet containing 0.9 g Kg-1 Se or the same diet supplemented with a Se-Yeast product (Sel-Plex) to achieve Se concentrations ranging from 1.5–8.9 g Kg-1 for a period of ten weeks. Fish were sampled every two weeks for analysis. The kinetics of Se bioaccumulation and the effects on fish selenoprotein expression was determined in different tissues combining chemical and bimolecular techniques.ResultsThe Sel-Plex enriched diets did not have any effect on survival and growth performance. The highest Se levels were found in liver and kidney followed by muscle and blood cells. Analysis of the Se concentration factor showed that liver is able to initially regulate the amount of Se accumulated. However, with higher dietary Se level (4.8 and 8.9 g Kg-1) and longer times of exposure (10 weeks), regulation is ineffective and the Se tissue concentration increases. The expression of the selected trout selenoprotein transcripts showed an inverse correlation with Sel-Plex augmentation in most cases. In liver, kidney and blood cells the highest up-regulation of the trout selenoprotein genes was seen mostly in the group fed the diet enriched with the lowest concentration of Sel-Plex (0.5 g Kg-1) for 10 weeks.ConclusionSel-Plex may represent an excellent Se supplement to deliver a high level of Se without provoking harm to the fish and to guarantee the maximal absorption of the element. According to our results, a dietary supplementation of Sel-Plex between 0.5 and 4 g Kg-1 may allow maximal benefits, whereas 8 g Kg-1 may be excessive for the purpose of supplementation.",
author = "Davide Pacitti and Lawan, {Muhammad M.} and John Sweetman and Martin, {Samuel A.M.} and J{\"o}rg Feldmann and Secombes, {Christopher J.}",
note = "Correction Published: February 10, 2016 Acknowledgements This study was supported by Alltech (ZY002 RGF0258) and the Principal’s Interdisciplinary Fund at the University of Aberdeen (award BL900.ROQ0061). Thanks go to the Hellenic Centre for Marine Research, which synthesized all the diets used in this experiment. D.P. carried out the experiment and performed all the molecular biology analysis, interpreted the results and drafted the manuscript. C.J.S and S.A.M.M. supervised the experiment, participating in the experimental design and revision of the manuscript. M.M.L. participated in the sampling and performed all the chemical analysis under the supervision of J.F. Pacitti D, Lawan MM, Sweetman J, Martin SAM, Feldmann J, Secombes CJ (2016) Correction: Selenium Supplementation in Fish: A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss). PLoS ONE 11(2): e0144681. doi:10.1371/journal.pone.0144681 The unit used to indicate Selenium concentration appears incorrectly throughout the manuscript. The correct unit is mg Kg-1. The values for Selenium concentrations provided as 0.5, 4, and 8 mg Kg-1 throughout the article are incorrect. The correct Selenium concentrations are 0.25, 2, and 4 mg Kg-1 respectively.",
year = "2015",
month = "5",
day = "15",
doi = "10.1371/journal.pone.0127041",
language = "English",
volume = "10",
journal = "PloS ONE",
issn = "1932-6203",
publisher = "PUBLIC LIBRARY SCIENCE",
number = "5",

}

TY - JOUR

T1 - Selenium Supplementation in Fish

T2 - A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss)

AU - Pacitti, Davide

AU - Lawan, Muhammad M.

AU - Sweetman, John

AU - Martin, Samuel A.M.

AU - Feldmann, Jörg

AU - Secombes, Christopher J.

N1 - Correction Published: February 10, 2016 Acknowledgements This study was supported by Alltech (ZY002 RGF0258) and the Principal’s Interdisciplinary Fund at the University of Aberdeen (award BL900.ROQ0061). Thanks go to the Hellenic Centre for Marine Research, which synthesized all the diets used in this experiment. D.P. carried out the experiment and performed all the molecular biology analysis, interpreted the results and drafted the manuscript. C.J.S and S.A.M.M. supervised the experiment, participating in the experimental design and revision of the manuscript. M.M.L. participated in the sampling and performed all the chemical analysis under the supervision of J.F. Pacitti D, Lawan MM, Sweetman J, Martin SAM, Feldmann J, Secombes CJ (2016) Correction: Selenium Supplementation in Fish: A Combined Chemical and Biomolecular Study to Understand Sel-Plex Assimilation and Impact on Selenoproteome Expression in Rainbow Trout (Oncorhynchus mykiss). PLoS ONE 11(2): e0144681. doi:10.1371/journal.pone.0144681 The unit used to indicate Selenium concentration appears incorrectly throughout the manuscript. The correct unit is mg Kg-1. The values for Selenium concentrations provided as 0.5, 4, and 8 mg Kg-1 throughout the article are incorrect. The correct Selenium concentrations are 0.25, 2, and 4 mg Kg-1 respectively.

PY - 2015/5/15

Y1 - 2015/5/15

N2 - BackgroundSelenium (Se) is an essential oligonutrient, as a component of several Se-containing proteins (selenoproteins), which exert important biological functions within an organism. In livestock, Se-enriched products have been proposed as dietary supplements to be included into functional feeds for animal preventive health care. To this end, it is important to understand the optimal range of concentrations for supplementation and how long it takes to be assimilated into the organism.MethodsIn this study, rainbow trout (Oncorhynchus mykiss) were fed a control diet containing 0.9 g Kg-1 Se or the same diet supplemented with a Se-Yeast product (Sel-Plex) to achieve Se concentrations ranging from 1.5–8.9 g Kg-1 for a period of ten weeks. Fish were sampled every two weeks for analysis. The kinetics of Se bioaccumulation and the effects on fish selenoprotein expression was determined in different tissues combining chemical and bimolecular techniques.ResultsThe Sel-Plex enriched diets did not have any effect on survival and growth performance. The highest Se levels were found in liver and kidney followed by muscle and blood cells. Analysis of the Se concentration factor showed that liver is able to initially regulate the amount of Se accumulated. However, with higher dietary Se level (4.8 and 8.9 g Kg-1) and longer times of exposure (10 weeks), regulation is ineffective and the Se tissue concentration increases. The expression of the selected trout selenoprotein transcripts showed an inverse correlation with Sel-Plex augmentation in most cases. In liver, kidney and blood cells the highest up-regulation of the trout selenoprotein genes was seen mostly in the group fed the diet enriched with the lowest concentration of Sel-Plex (0.5 g Kg-1) for 10 weeks.ConclusionSel-Plex may represent an excellent Se supplement to deliver a high level of Se without provoking harm to the fish and to guarantee the maximal absorption of the element. According to our results, a dietary supplementation of Sel-Plex between 0.5 and 4 g Kg-1 may allow maximal benefits, whereas 8 g Kg-1 may be excessive for the purpose of supplementation.

AB - BackgroundSelenium (Se) is an essential oligonutrient, as a component of several Se-containing proteins (selenoproteins), which exert important biological functions within an organism. In livestock, Se-enriched products have been proposed as dietary supplements to be included into functional feeds for animal preventive health care. To this end, it is important to understand the optimal range of concentrations for supplementation and how long it takes to be assimilated into the organism.MethodsIn this study, rainbow trout (Oncorhynchus mykiss) were fed a control diet containing 0.9 g Kg-1 Se or the same diet supplemented with a Se-Yeast product (Sel-Plex) to achieve Se concentrations ranging from 1.5–8.9 g Kg-1 for a period of ten weeks. Fish were sampled every two weeks for analysis. The kinetics of Se bioaccumulation and the effects on fish selenoprotein expression was determined in different tissues combining chemical and bimolecular techniques.ResultsThe Sel-Plex enriched diets did not have any effect on survival and growth performance. The highest Se levels were found in liver and kidney followed by muscle and blood cells. Analysis of the Se concentration factor showed that liver is able to initially regulate the amount of Se accumulated. However, with higher dietary Se level (4.8 and 8.9 g Kg-1) and longer times of exposure (10 weeks), regulation is ineffective and the Se tissue concentration increases. The expression of the selected trout selenoprotein transcripts showed an inverse correlation with Sel-Plex augmentation in most cases. In liver, kidney and blood cells the highest up-regulation of the trout selenoprotein genes was seen mostly in the group fed the diet enriched with the lowest concentration of Sel-Plex (0.5 g Kg-1) for 10 weeks.ConclusionSel-Plex may represent an excellent Se supplement to deliver a high level of Se without provoking harm to the fish and to guarantee the maximal absorption of the element. According to our results, a dietary supplementation of Sel-Plex between 0.5 and 4 g Kg-1 may allow maximal benefits, whereas 8 g Kg-1 may be excessive for the purpose of supplementation.

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DO - 10.1371/journal.pone.0127041

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