Selenoprotein expression in endothelial cells from different human vasculature and species

S Miller, S W Walker, John Arthur, M H Lewin, Karen Ross, Fergus Nicol, A F Howie, G J Beckett

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Selenium (Se) can protect endothelial cells (EC) from oxidative damage by altering the expression of selenoproteins with antioxidant function such as cytoplasmic glutathione peroxidase (cyGPX), phospholipid hydroperoxide glutathione peroxidase (PHGPX) and thioredoxin reductase (TR). If the role of Se on EC function is to be studied, it is essential that a model system be chosen which reflects selenoprotein expression in human EC derived from vessels prone to developing atheroma. We have used [Se-75]-selenite labelling and selenoenzyme measurements to compare the selenoproteins expressed by cultures of EC isolated from different human vasculature with EC isolated from bovine and porcine aorta. Only small differences were observed in selenoprotein expression and activity in EC originating from human coronary artery, human umbilical vein (HUVEC), human umbilical artery and the human EC line EAhy926. The selenoprotein profile in HUVEC was consistent over eight passages and HUVEC isolated from four cords also showed little variability. In contrast, EC isolated from pig and bovine aorta showed marked differences in selenoprotein expression when compared to human cells. This study firmly establishes the suitability and consistency of using HUVEC (and possibly the human cell line EAhy926) as a model to study the effects of Se on EC function in relation to atheroma development in the coronary artery. Bovine or porcine EC appear to be an inappropriate model. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)85-93
Number of pages9
JournalBiochimica et Biophysica Acta. Molecular Basis of Disease : BBA
Volume1588
Issue number1
Publication statusPublished - 9 Oct 2002

Keywords

  • selenium
  • atherosclerosis
  • endothelium
  • selenoprotein
  • LOW-DENSITY-LIPOPROTEIN
  • PROTEIN-KINASE-C
  • GLUTATHIONE-PEROXIDASE
  • THIOREDOXIN REDUCTASE
  • CULTURE
  • IDENTIFICATION
  • HYPEROXIA
  • BINDING
  • RELEASE
  • GROWTH

Cite this

Miller, S., Walker, S. W., Arthur, J., Lewin, M. H., Ross, K., Nicol, F., ... Beckett, G. J. (2002). Selenoprotein expression in endothelial cells from different human vasculature and species. Biochimica et Biophysica Acta. Molecular Basis of Disease : BBA , 1588(1), 85-93.

Selenoprotein expression in endothelial cells from different human vasculature and species. / Miller, S ; Walker, S W ; Arthur, John; Lewin, M H ; Ross, Karen; Nicol, Fergus; Howie, A F ; Beckett, G J .

In: Biochimica et Biophysica Acta. Molecular Basis of Disease : BBA , Vol. 1588, No. 1, 09.10.2002, p. 85-93.

Research output: Contribution to journalArticle

Miller, S, Walker, SW, Arthur, J, Lewin, MH, Ross, K, Nicol, F, Howie, AF & Beckett, GJ 2002, 'Selenoprotein expression in endothelial cells from different human vasculature and species', Biochimica et Biophysica Acta. Molecular Basis of Disease : BBA , vol. 1588, no. 1, pp. 85-93.
Miller, S ; Walker, S W ; Arthur, John ; Lewin, M H ; Ross, Karen ; Nicol, Fergus ; Howie, A F ; Beckett, G J . / Selenoprotein expression in endothelial cells from different human vasculature and species. In: Biochimica et Biophysica Acta. Molecular Basis of Disease : BBA . 2002 ; Vol. 1588, No. 1. pp. 85-93.
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