TY - JOUR
T1 - Self-Powered Wireless Carbohydrate/Oxygen Sensitive Biodevice Based on Radio Signal Transmission
AU - Falk, Magnus
AU - Alcalde, Miguel
AU - Bartlett, Philip N.
AU - De Lacey, Antonio L.
AU - Gorton, Lo
AU - Gutierrez-Sanchez, Cristina
AU - Haddad, Raoudha
AU - Kilburn, Jeremy
AU - Leech, Donal
AU - Ludwig, Roland
AU - Magner, Edmond
AU - Mate, Diana M.
AU - Conghaile, Peter O.
AU - Ortiz, Roberto
AU - Pita, Marcos
AU - Poller, Sascha
AU - Ruzgas, Tautgirdas
AU - Salaj-Kosla, Urszula
AU - Schuhmann, Wolfgang
AU - Sebelius, Fredrik
AU - Shao, Minling
AU - Stoica, Leonard
AU - Sygmund, Cristoph
AU - Tilly, Jonas
AU - Toscano, Miguel D.
AU - Vivekananthan, Jeevanthi
AU - Wright, Emma
AU - Shleev, Sergey
N1 - The research was undertaken as part of a ‘‘3D-nanobiodevice’’ consortium (www.mah.se/3dnanobiodevice) organized and funded under the EU FP7
NMP program. Co-authors Fredrik Sebelius and Jonas Tilly are employed by Novosense AB. Novosense AB provided support in the form of salaries for authors Fredrik Sebelius and Jonas Tilly, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the author contributions section. Co-author Miguel D. Toscano is employed by Novozymes A/S. Novozymes A/S provided support in the form of salary for author Miguel D. Toscano, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific role of this author is articulated in the author contributions section.
PY - 2014/10/13
Y1 - 2014/10/13
N2 - Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, we demonstrate the operation of self-sustained carbohydrate and oxygen sensitive biodevices, consisting of a wireless electronic unit, radio transmitter and separate sensing bioelectrodes, supplied with electrical energy from a combined multi-enzyme fuel cell generating sufficient current at required voltage to power the electronics. A carbohydrate/oxygen enzymatic fuel cell was assembled by comparing the performance of a range of different bioelectrodes followed by selection of the most suitable, stable combination. Carbohydrates (viz. lactose for the demonstration) and oxygen were also chosen as bioanalytes, being important biomarkers, to demonstrate the operation of the self-contained biosensing device, employing enzyme-modified bioelectrodes to enable the actual sensing. A wireless electronic unit, consisting of a micropotentiostat, an energy harvesting module (voltage amplifier together with a capacitor), and a radio microchip, were designed to enable the biofuel cell to be used as a power supply for managing the sensing devices and for wireless data transmission. The electronic system used required current and voltages greater than 44 mu A and 0.57 V, respectively to operate; which the biofuel cell was capable of providing, when placed in a carbohydrate and oxygen containing buffer. In addition, a USB based receiver and computer software were employed for proof-of concept tests of the developed biodevices. Operation of bench-top prototypes was demonstrated in buffers containing different concentrations of the analytes, showcasing that the variation in response of both carbohydrate and oxygen biosensors could be monitored wirelessly in real-time as analyte concentrations in buffers were changed, using only an enzymatic fuel cell as a power supply.
AB - Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, we demonstrate the operation of self-sustained carbohydrate and oxygen sensitive biodevices, consisting of a wireless electronic unit, radio transmitter and separate sensing bioelectrodes, supplied with electrical energy from a combined multi-enzyme fuel cell generating sufficient current at required voltage to power the electronics. A carbohydrate/oxygen enzymatic fuel cell was assembled by comparing the performance of a range of different bioelectrodes followed by selection of the most suitable, stable combination. Carbohydrates (viz. lactose for the demonstration) and oxygen were also chosen as bioanalytes, being important biomarkers, to demonstrate the operation of the self-contained biosensing device, employing enzyme-modified bioelectrodes to enable the actual sensing. A wireless electronic unit, consisting of a micropotentiostat, an energy harvesting module (voltage amplifier together with a capacitor), and a radio microchip, were designed to enable the biofuel cell to be used as a power supply for managing the sensing devices and for wireless data transmission. The electronic system used required current and voltages greater than 44 mu A and 0.57 V, respectively to operate; which the biofuel cell was capable of providing, when placed in a carbohydrate and oxygen containing buffer. In addition, a USB based receiver and computer software were employed for proof-of concept tests of the developed biodevices. Operation of bench-top prototypes was demonstrated in buffers containing different concentrations of the analytes, showcasing that the variation in response of both carbohydrate and oxygen biosensors could be monitored wirelessly in real-time as analyte concentrations in buffers were changed, using only an enzymatic fuel cell as a power supply.
U2 - 10.1371/journal.pone.0109104
DO - 10.1371/journal.pone.0109104
M3 - Article
VL - 9
SP - 1
EP - 9
JO - PloS ONE
JF - PloS ONE
SN - 1932-6203
IS - 10
M1 - 109104
ER -