Separation and identification of hen egg protein isoforms using SDS-PAGE and 2D gel electrophoresis with MALDI-TOF mass spectrometry

Vassilios Raikos, Rasmus Hansen, Lydia Campbell, Stephen R. Euston

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Knowledge of the chemical composition and physicochemical properties of native egg white and yolk is necessary to interpret the functional and biological properties attributed to specific egg components. To date, many of the proteins located in this complex biological fluid remain uncharacterised, if not unknown. High-resolution techniques for proteome analysis, including SDS-PAGE and 2-dimensional (2D) gel electrophoresis, combined with mass spectrometry, were employed to separate and identify several protein components in hen's egg. An advanced and sensitive glycoprotein staining kit was used to detect the presence of glycosylated proteins in the egg samples. Numerous spots were revealed when a mixture of egg white and yolk was subjected to 2D gel electrophoresis. Several of the already known egg proteins were identified. Isoforms of ovalbumin and conalbumin were visualised. The presence of FLJ 10305 and Fatso proteins in the proteome of Gallus domesticus was also confirmed.
Original languageEnglish
Pages (from-to)702-710
Number of pages9
JournalFood Chemistry
Volume99
Issue number4
Early online date2 Nov 2005
DOIs
Publication statusPublished - 2006

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Egg Proteins
protein isoforms
egg proteins
matrix-assisted laser desorption-ionization mass spectrometry
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis
gel electrophoresis
Mass spectrometry
polyacrylamide gel electrophoresis
hens
Polyacrylamide Gel Electrophoresis
Mass Spectrometry
Egg White
Protein Isoforms
Egg Yolk
Gels
Proteome
egg albumen
proteome
egg yolk

Keywords

  • hen egg proteins
  • SDS-PAGE
  • 2-dimensional gel electrophoresis
  • glycoprotein stain kit
  • MALDI-TOF

Cite this

Separation and identification of hen egg protein isoforms using SDS-PAGE and 2D gel electrophoresis with MALDI-TOF mass spectrometry. / Raikos, Vassilios; Hansen, Rasmus; Campbell, Lydia; Euston, Stephen R.

In: Food Chemistry, Vol. 99, No. 4, 2006, p. 702-710.

Research output: Contribution to journalArticle

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