SEPARATION OF METALLOTHIONEIN ISOFORMS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

J H BEATTIE, M P RICHARDS

    Research output: Contribution to journalComment/debate

    38 Citations (Scopus)

    Abstract

    Current techniques for the separation and quantification of metallothionein isoforms have limited value for routine analysis. Isoforms having a similar charge have been separated successfully using reversed-phase HPLC but this technique suffers from a slow sample turnover time. The use of the surfactant sodium dodecyl sulphate for the separation of metallothionein isoforms by micellar electrokinetic capillary chromatography (MECC) is described. The charge-different isoforms MT-1 and MT-2 from rats, rabbits and sheep were separated within 9-12 min. In addition, a varying degree of heterogeneity was observed in purified samples of human MT-1, rat MT-2, rabbit MT-1, rabbit MT-2 and sheep MT-1. The behaviour of chicken MT was different from that of any other species. The separation of sheep liver extracts indicated the potential of MECC as the basis for a quantitative assay for both charge-different and charge-similar metallothionein isoforms.

    Original languageEnglish
    Pages (from-to)129-134
    Number of pages6
    JournalJournal of Chromatography A
    Volume664
    Issue number1
    Publication statusPublished - 25 Mar 1994

    Keywords

    • PERFORMANCE LIQUID-CHROMATOGRAPHY
    • RAT-LIVER
    • ZONE ELECTROPHORESIS
    • QUANTIFICATION
    • INDUCTION
    • CADMIUM
    • ASSAY
    • GENES
    • CELLS

    Cite this

    SEPARATION OF METALLOTHIONEIN ISOFORMS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY. / BEATTIE, J H ; RICHARDS, M P .

    In: Journal of Chromatography A, Vol. 664, No. 1, 25.03.1994, p. 129-134.

    Research output: Contribution to journalComment/debate

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    title = "SEPARATION OF METALLOTHIONEIN ISOFORMS BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY",
    abstract = "Current techniques for the separation and quantification of metallothionein isoforms have limited value for routine analysis. Isoforms having a similar charge have been separated successfully using reversed-phase HPLC but this technique suffers from a slow sample turnover time. The use of the surfactant sodium dodecyl sulphate for the separation of metallothionein isoforms by micellar electrokinetic capillary chromatography (MECC) is described. The charge-different isoforms MT-1 and MT-2 from rats, rabbits and sheep were separated within 9-12 min. In addition, a varying degree of heterogeneity was observed in purified samples of human MT-1, rat MT-2, rabbit MT-1, rabbit MT-2 and sheep MT-1. The behaviour of chicken MT was different from that of any other species. The separation of sheep liver extracts indicated the potential of MECC as the basis for a quantitative assay for both charge-different and charge-similar metallothionein isoforms.",
    keywords = "PERFORMANCE LIQUID-CHROMATOGRAPHY, RAT-LIVER, ZONE ELECTROPHORESIS, QUANTIFICATION, INDUCTION, CADMIUM, ASSAY, GENES, CELLS",
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    AU - RICHARDS, M P

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    AB - Current techniques for the separation and quantification of metallothionein isoforms have limited value for routine analysis. Isoforms having a similar charge have been separated successfully using reversed-phase HPLC but this technique suffers from a slow sample turnover time. The use of the surfactant sodium dodecyl sulphate for the separation of metallothionein isoforms by micellar electrokinetic capillary chromatography (MECC) is described. The charge-different isoforms MT-1 and MT-2 from rats, rabbits and sheep were separated within 9-12 min. In addition, a varying degree of heterogeneity was observed in purified samples of human MT-1, rat MT-2, rabbit MT-1, rabbit MT-2 and sheep MT-1. The behaviour of chicken MT was different from that of any other species. The separation of sheep liver extracts indicated the potential of MECC as the basis for a quantitative assay for both charge-different and charge-similar metallothionein isoforms.

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    KW - CELLS

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