Sequence diversity and virulence in Zea mays of Maize streak virus isolates

D P Martin, J A Willment, R Billharz, R Velders, B Odhiambo, J Njuguna, D James, E P Rybicki

Research output: Contribution to journalArticle

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Abstract

Full genomic sequences were determined for 12 Maize streak virus (MSV) isolates obtained from Zea mays and wild grass species. These and 10 other publicly available full-length sequences were used to classify a total of 66 additional MSV isolates that had been characterized by PCR-restriction fragment length polymorphism and/or partial nucleotide sequence analysis. A description is given of the host and geographical distribution of the MSV strain and subtype groupings identified. The relationship between the genotypes of 21 fully sequenced virus isolates and their virulence in differentially MSV-resistant Z. mays genotypes was examined. Within the only MSV strain grouping that produced severe symptoms in maize, highly virulent and widely distributed genotypes were identified that are likely to pose the most serious threat to maize production in Africa. Evidence is presented that certain of the isolates investigated may be the products of either intra- or interspecific recombination.
Original languageEnglish
Pages (from-to)247-55
Number of pages9
JournalJournal of General Virology
Volume288
Issue number2
DOIs
Publication statusPublished - 2001

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Maize streak virus
Zea mays
Virulence
Genotype
Horizontal Gene Transfer
Poaceae
Restriction Fragment Length Polymorphisms
Sequence Analysis
Viruses
Polymerase Chain Reaction

Keywords

  • Geminiviridae
  • Genetic Variation
  • Genome, Viral
  • Recombination, Genetic
  • Sequence Analysis, DNA
  • Virulence
  • Zea mays

Cite this

Martin, D. P., Willment, J. A., Billharz, R., Velders, R., Odhiambo, B., Njuguna, J., ... Rybicki, E. P. (2001). Sequence diversity and virulence in Zea mays of Maize streak virus isolates. Journal of General Virology, 288(2), 247-55. https://doi.org/10.1006/viro.2001.1075

Sequence diversity and virulence in Zea mays of Maize streak virus isolates. / Martin, D P; Willment, J A; Billharz, R; Velders, R; Odhiambo, B; Njuguna, J; James, D; Rybicki, E P.

In: Journal of General Virology, Vol. 288, No. 2, 2001, p. 247-55.

Research output: Contribution to journalArticle

Martin, DP, Willment, JA, Billharz, R, Velders, R, Odhiambo, B, Njuguna, J, James, D & Rybicki, EP 2001, 'Sequence diversity and virulence in Zea mays of Maize streak virus isolates', Journal of General Virology, vol. 288, no. 2, pp. 247-55. https://doi.org/10.1006/viro.2001.1075
Martin DP, Willment JA, Billharz R, Velders R, Odhiambo B, Njuguna J et al. Sequence diversity and virulence in Zea mays of Maize streak virus isolates. Journal of General Virology. 2001;288(2):247-55. https://doi.org/10.1006/viro.2001.1075
Martin, D P ; Willment, J A ; Billharz, R ; Velders, R ; Odhiambo, B ; Njuguna, J ; James, D ; Rybicki, E P. / Sequence diversity and virulence in Zea mays of Maize streak virus isolates. In: Journal of General Virology. 2001 ; Vol. 288, No. 2. pp. 247-55.
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