SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

C Cunningham, Carol A McPherson, Jennifer Martin, William J. Harris, Harry James Flint

Research output: Contribution to journalComment/debate

18 Citations (Scopus)

Abstract

A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

Original languageEnglish
Pages (from-to)320-323
Number of pages4
JournalMolecular & general genetics
Volume228
Issue number1-2
DOIs
Publication statusPublished - Aug 1991

Keywords

  • CELLULASE GENE SEQUENCE
  • RUMINOCOCCUS-FLAVEFACIENS
  • RUMEN BACTERIA
  • ENDOGLUCANASE GENE
  • CELLULASE FAMILIES
  • NUCLEOTIDE-SEQUENCE
  • MOLECULAR-CLONING
  • ESCHERICHIA-COLI
  • FD-1
  • CELA

Cite this

SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17. / Cunningham, C ; McPherson, Carol A ; Martin, Jennifer ; Harris, William J.; Flint, Harry James.

In: Molecular & general genetics, Vol. 228, No. 1-2, 08.1991, p. 320-323.

Research output: Contribution to journalComment/debate

Cunningham, C ; McPherson, Carol A ; Martin, Jennifer ; Harris, William J. ; Flint, Harry James. / SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17. In: Molecular & general genetics. 1991 ; Vol. 228, No. 1-2. pp. 320-323.
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AU - Harris, William J.

AU - Flint, Harry James

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AB - A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

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KW - CELLULASE FAMILIES

KW - NUCLEOTIDE-SEQUENCE

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KW - ESCHERICHIA-COLI

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KW - CELA

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