SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

C  Cunningham; Carol A  McPherson; Jennifer  Martin; William J. Harris; Harry James Flint

doi:10.1007/BF00282484

SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

C Cunningham, Carol A McPherson, Jennifer Martin, William J. Harris, Harry James Flint

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Comment/debate › peer-review

19 Citations (Scopus)

Abstract

A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

Original language	English
Pages (from-to)	320-323
Number of pages	4
Journal	Molecular & general genetics
Volume	228
Issue number	1-2
DOIs	https://doi.org/10.1007/BF00282484
Publication status	Published - Aug 1991

Keywords

CELLULASE GENE SEQUENCE
RUMINOCOCCUS-FLAVEFACIENS
RUMEN BACTERIA
ENDOGLUCANASE GENE
CELLULASE FAMILIES
NUCLEOTIDE-SEQUENCE
MOLECULAR-CLONING
ESCHERICHIA-COLI
FD-1
CELA

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https://link.springer.com/article/10.1007/BF00282484

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@article{fb06842b9fa74237a070bbbef9e75343,

title = "SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17",

abstract = "A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.",

keywords = "CELLULASE GENE SEQUENCE, RUMINOCOCCUS-FLAVEFACIENS, RUMEN BACTERIA, ENDOGLUCANASE GENE, CELLULASE FAMILIES, NUCLEOTIDE-SEQUENCE, MOLECULAR-CLONING, ESCHERICHIA-COLI, FD-1, CELA",

author = "C Cunningham and McPherson, {Carol A} and Jennifer Martin and Harris, {William J.} and Flint, {Harry James}",

year = "1991",

month = aug,

doi = "10.1007/BF00282484",

language = "English",

volume = "228",

pages = "320--323",

journal = "Molecular & general genetics",

issn = "0026-8925",

publisher = "Springer Verlag",

number = "1-2",

}

TY - JOUR

T1 - SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

AU - Cunningham, C

AU - McPherson, Carol A

AU - Martin, Jennifer

AU - Harris, William J.

AU - Flint, Harry James

PY - 1991/8

Y1 - 1991/8

N2 - A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

AB - A cellulase gene (endA) was isolated from a library of Ruminococcus flavefaciens strain 17 DNA fragments inserted in pUC13. The endA product showed activity against acid-swollen cellulose, carboxymethylcellulose, lichenan, cellopentaose and cellotetraose, but showed no activity against cellotriose or binding to avicel. Nucleotide sequencing indicated an encoded product of 455 amino acids which showed significant sequence similarity (ranging from 56% to 61%) with three endoglucanases from Ruminococcus albus, and with Clostridium thermocellum endoglucanase E. Little relatedness was found with a cellodextrinase previously isolated from R. flavefaciens FD1.

KW - CELLULASE GENE SEQUENCE

KW - RUMINOCOCCUS-FLAVEFACIENS

KW - RUMEN BACTERIA

KW - ENDOGLUCANASE GENE

KW - CELLULASE FAMILIES

KW - NUCLEOTIDE-SEQUENCE

KW - MOLECULAR-CLONING

KW - ESCHERICHIA-COLI

KW - FD-1

KW - CELA

U2 - 10.1007/BF00282484

DO - 10.1007/BF00282484

M3 - Comment/debate

VL - 228

SP - 320

EP - 323

JO - Molecular & general genetics

JF - Molecular & general genetics

SN - 0026-8925

IS - 1-2

ER -

SEQUENCE OF A CELLULASE GENE FROM THE RUMEN ANAEROBE RUMINOCOCCUS-FLAVEFACIENS 17

Abstract

Keywords

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