Serotonin potentiates the response of neurons of the superficial laminae of the rat spinal dorsal horn to gamma-aminobutyric acid

H Li, B Lang, J F Kang, Y Q Li

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Employing the Nystatin-perforated whole-cell patch-clamp recording technique, the modulatory effects of serotonin (5-HT) on gamma-aminobutyric acid (GABA)-activated whole-cell currents were investigated in neurons acutely dissociated from the superficial laminae (laminae I and II) of the rat spinal dorsal horn. The results showed: (1) GABA acted on GABA(A) receptors and elicited inward Cl(-) currents (I(GABA)) at a holding potential (V(H)) of -40 mV; (2) 5-HT potentiated GABA-induced Cl(-) current without affecting the reversal potential of I(GABA) and the apparent affinity of GABA to its receptor; (3) alpha-methyl-5-HT, a selective agonist of 5-HT(2) receptor, mimicked the potentiation effect of 5-HT on I(GABA), whereas ketanserine, an antagonist of 5-HT(2) receptor, blocked the potentiation effect of 5-HT; (4) Chelerythrine, an inhibitor of protein kinase C, reduced the potentiation effect of 5-HT on I(GABA). The present results indicate: (1) The potentiation of 5-HT on I(GABA) is mediated by 5-HT(2) receptor and through a protein kinase-dependent transduction pathway; (2) The interactions between 5-HT and GABA might play an important role in the modulation of nociceptive information transmission at spinal cord level.
Original languageEnglish
Pages (from-to)559-65
Number of pages7
JournalBrain Research Bulletin
Volume52
Issue number6
Publication statusPublished - 2000

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gamma-Aminobutyric Acid
Serotonin
Neurons
Serotonin 5-HT2 Receptors
Substantia Gelatinosa
Spinal Cord Dorsal Horn
Nystatin
GABA Receptors
Patch-Clamp Techniques
Protein Kinases
Protein Kinase C
Spinal Cord

Keywords

  • Animals
  • Cells, Cultured
  • Drug Interactions
  • Membrane Potentials
  • Posterior Horn Cells
  • Protein Kinase C
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Serotonin
  • Serotonin
  • Signal Transduction
  • gamma-Aminobutyric Acid

Cite this

Serotonin potentiates the response of neurons of the superficial laminae of the rat spinal dorsal horn to gamma-aminobutyric acid. / Li, H; Lang, B; Kang, J F; Li, Y Q.

In: Brain Research Bulletin, Vol. 52, No. 6, 2000, p. 559-65.

Research output: Contribution to journalArticle

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AB - Employing the Nystatin-perforated whole-cell patch-clamp recording technique, the modulatory effects of serotonin (5-HT) on gamma-aminobutyric acid (GABA)-activated whole-cell currents were investigated in neurons acutely dissociated from the superficial laminae (laminae I and II) of the rat spinal dorsal horn. The results showed: (1) GABA acted on GABA(A) receptors and elicited inward Cl(-) currents (I(GABA)) at a holding potential (V(H)) of -40 mV; (2) 5-HT potentiated GABA-induced Cl(-) current without affecting the reversal potential of I(GABA) and the apparent affinity of GABA to its receptor; (3) alpha-methyl-5-HT, a selective agonist of 5-HT(2) receptor, mimicked the potentiation effect of 5-HT on I(GABA), whereas ketanserine, an antagonist of 5-HT(2) receptor, blocked the potentiation effect of 5-HT; (4) Chelerythrine, an inhibitor of protein kinase C, reduced the potentiation effect of 5-HT on I(GABA). The present results indicate: (1) The potentiation of 5-HT on I(GABA) is mediated by 5-HT(2) receptor and through a protein kinase-dependent transduction pathway; (2) The interactions between 5-HT and GABA might play an important role in the modulation of nociceptive information transmission at spinal cord level.

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KW - Posterior Horn Cells

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