Serum carotenoids and oxidative DNA damage in human lymphocytes

A R Collins, B Olmedilla, S Southon, F Granado, Susan Joyce Duthie

    Research output: Contribution to journalArticle

    139 Citations (Scopus)

    Abstract

    Carotenoids are thought to act as antioxidants in vivo, decreasing oxidative damage to biomolecules and thus protecting against coronary heart disease and cancer. However, human intervention studies with p-carotene have given equivocal results in terms of cancer incidence. In an alternative molecular epidemiological approach, we have employed the 'comet assay' (single cell alkaline gel electrophoresis) to measure strand breaks, oxidized pyrimidines and altered purines in the DNA of lymphocytes from volunteers supplemented with alp-carotene, lutein, lycopene or placebo. In addition, we measured concentrations of the main serum carotenoids, and vitamins E and C, by HPLC. We report a significant negative correlation between basal concentrations of total serum carotenoids and oxidized pyrimidines. A similar correlation was seen between individual carotenoids (notably lutein and p-carotene) and oxidized pyrimidines. However, carotenoid supplementation did not have a significant effect on endogenous oxidative damage. This suggests that there are some factors in the basal diet, probably found in fruit and vegetables, that decrease oxidative damage to DNA, In this case, basal serum carotenoids may simply be markers of consumption of fruit and vegetables, they themselves having little or no protective value.

    Original languageEnglish
    Pages (from-to)2159-2162
    Number of pages4
    JournalCarcinogenesis
    Volume19
    Issue number12
    DOIs
    Publication statusPublished - Dec 1998

    Keywords

    • BETA-CAROTENE
    • CARDIOVASCULAR-DISEASE
    • ESCHERICHIA-COLI
    • HUMAN-CELLS
    • SUPPLEMENTATION
    • CANCER
    • PYRIMIDINES
    • POPULATION
    • TOCOPHEROL

    Cite this

    Collins, A. R., Olmedilla, B., Southon, S., Granado, F., & Duthie, S. J. (1998). Serum carotenoids and oxidative DNA damage in human lymphocytes. Carcinogenesis, 19(12), 2159-2162. https://doi.org/10.1093/carcin/19.12.2159

    Serum carotenoids and oxidative DNA damage in human lymphocytes. / Collins, A R ; Olmedilla, B ; Southon, S ; Granado, F ; Duthie, Susan Joyce.

    In: Carcinogenesis, Vol. 19, No. 12, 12.1998, p. 2159-2162.

    Research output: Contribution to journalArticle

    Collins, AR, Olmedilla, B, Southon, S, Granado, F & Duthie, SJ 1998, 'Serum carotenoids and oxidative DNA damage in human lymphocytes' Carcinogenesis, vol. 19, no. 12, pp. 2159-2162. https://doi.org/10.1093/carcin/19.12.2159
    Collins AR, Olmedilla B, Southon S, Granado F, Duthie SJ. Serum carotenoids and oxidative DNA damage in human lymphocytes. Carcinogenesis. 1998 Dec;19(12):2159-2162. https://doi.org/10.1093/carcin/19.12.2159
    Collins, A R ; Olmedilla, B ; Southon, S ; Granado, F ; Duthie, Susan Joyce. / Serum carotenoids and oxidative DNA damage in human lymphocytes. In: Carcinogenesis. 1998 ; Vol. 19, No. 12. pp. 2159-2162.
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