Serum opsonin ficolin-A enhances host–fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge

Stefan Bidula, Darren W. Sexton, Silke Schelenz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Invasive aspergillosis is a devastating invasive fungal disease associated with a high mortality rate in the immunocompromised, such as leukaemia patients, transplant patients and those with HIV/AIDS. The rodent serum orthologue of human L-ficolin, ficolin-A, can bind to and opsonize Aspergillus fumigatus, the pathogen that causes invasive aspergillosis, and may participate in fungal defence. Using human monocyte-derived macrophages and neutrophils isolated from healthy donors, we investigated conidial association and fungal viability by flow cytometry and microscopy. Additionally, cytokine production was measured via cytometric bead arrays. Ficolin-A opsonization was observed to significantly enhance association of conidia, while also inhibiting hyphal growth and contributing to increased fungal killing following incubation with monocyte-derived macrophages and neutrophils. Additionally, ficolin-A opsonization was capable of manifesting a decrease in IL-8, IL-1β, IL-6, IL-10 and TNF-α production from MDM and IL-1β, IL-6 and TNF-α from neutrophils 24 h post-infection. In conclusion, rodent ficolin-A is functionally comparable to human L-ficolin and is capable of modulating the innate immune response to A. fumigatus, down-regulating cytokine production and could play an important role in airway immunity.
Original languageEnglish
Pages (from-to)133-142
Number of pages10
JournalMedical Microbiology and Immunology
Volume205
Issue number2
Early online date4 Sep 2015
DOIs
Publication statusPublished - 1 Apr 2016

Fingerprint

Opsonin Proteins
Aspergillus fumigatus
Neutrophils
Macrophages
Cytokines
Serum
Aspergillosis
Interleukin-1
Rodentia
Interleukin-6
Fungal Spores
Mycoses
Interleukin-8
Innate Immunity
Interleukin-10
ficolin
Microscopy
Immunity
Flow Cytometry
Leukemia

Keywords

  • aspergillosis
  • macrophage
  • neutrophil
  • cytokines
  • innate immunity

Cite this

Serum opsonin ficolin-A enhances host–fungal interactions and modulates cytokine expression from human monocyte-derived macrophages and neutrophils following Aspergillus fumigatus challenge. / Bidula, Stefan; Sexton, Darren W.; Schelenz, Silke.

In: Medical Microbiology and Immunology, Vol. 205, No. 2, 01.04.2016, p. 133-142.

Research output: Contribution to journalArticle

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