Seven strains of mice as potential models of bovine pasteurellosis following intranasal challenge with a bovine pneumonic strain of Pasteurella multocida A:3; comparisons of disease and pathological outcomes

J C Hodgson (Corresponding Author), M P Dagleish, L Gibbard, C W Bayne, J Finlayson, G M Moon, M Nath

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The gram-negative bacterium Pasteurella multocida causes pneumonic and systemic pasteurellosis in bovids for which vaccines are either unavailable or inadequate. The work assessed whether an intranasal P. multocida challenge in mice might provide a model of infection for future vaccine development work. Clinical, pathological and biochemical responses were compared in seven strains of mice challenged with a virulent bovine pneumonic isolate of P. multocida A:3. Six mouse strains (Porton, CD-1, BALB/c, VM, C57BL/10 and C57BL/6) developed clinical signs of pneumonic disease and variable pneumonic lesions 41-70 h post-infection. In contrast, mouse strain RIII became septicaemic within 36 h post-infection. Concentrations of plasma acute phase proteins and serum lipopolysaccharide increased in all mice after infection, and the main or interaction effect of mouse strain and infection status was statistically significant (P<0.05). Responses in C57BL/10 mice showed close similarity to bovine pneumonic and in RIII mice to bovine systemic pasteurellosis.

Original languageEnglish
Pages (from-to)634-640
Number of pages7
JournalResearch in Veterinary Science
Volume94
Issue number3
Early online date14 Feb 2013
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Pasteurella Infections
pasteurellosis
Pasteurella multocida
lungs
Lung
cattle
mice
Infection
infection
Pneumonic Pasteurellosis
Vaccines
Acute-Phase Proteins
acute phase proteins
Gram-Negative Bacteria
Inbred C57BL Mouse
vaccine development
Lung Diseases
Lipopolysaccharides
Blood Proteins
Gram-negative bacteria

Keywords

  • Pasteurellosis
  • Bovids
  • Mouse models
  • Endotoxaemia
  • Acute phase proteins
  • Pathology

Cite this

Seven strains of mice as potential models of bovine pasteurellosis following intranasal challenge with a bovine pneumonic strain of Pasteurella multocida A:3; comparisons of disease and pathological outcomes. / Hodgson, J C (Corresponding Author); Dagleish, M P; Gibbard, L; Bayne, C W; Finlayson, J; Moon, G M; Nath, M.

In: Research in Veterinary Science, Vol. 94, No. 3, 06.2013, p. 634-640.

Research output: Contribution to journalArticle

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abstract = "The gram-negative bacterium Pasteurella multocida causes pneumonic and systemic pasteurellosis in bovids for which vaccines are either unavailable or inadequate. The work assessed whether an intranasal P. multocida challenge in mice might provide a model of infection for future vaccine development work. Clinical, pathological and biochemical responses were compared in seven strains of mice challenged with a virulent bovine pneumonic isolate of P. multocida A:3. Six mouse strains (Porton, CD-1, BALB/c, VM, C57BL/10 and C57BL/6) developed clinical signs of pneumonic disease and variable pneumonic lesions 41-70 h post-infection. In contrast, mouse strain RIII became septicaemic within 36 h post-infection. Concentrations of plasma acute phase proteins and serum lipopolysaccharide increased in all mice after infection, and the main or interaction effect of mouse strain and infection status was statistically significant (P<0.05). Responses in C57BL/10 mice showed close similarity to bovine pneumonic and in RIII mice to bovine systemic pasteurellosis.",
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