Sex differences in the associations between L-arginine pathway metabolites, skeletal muscle mass and function, and their responses to resistance exercise, in old age

Mariasole Da Boit, S Tommasi, D Elliot, A Zinellu, S Sotgia, R Sibson, J Meakin, R M Aspden, Ciriaco Carru, A A Mangoni, Stuart R Gray

Research output: Contribution to journalArticle

2 Citations (Scopus)
5 Downloads (Pure)

Abstract

Objectives: The current study was designed to explore the associations between L-arginine metabolites and muscle mass and function in old age, which are largely unknown.
Design: The study used a randomised, double-blind, placebo-controlled design.
Setting: The study was carried out in a laboratory setting.
Participants: 50 healthy older adults [median age 70 years (IQR 67-73); 27 males].
Intervention: Participants undertook an 18-week resistance exercise program, and a nutritional intervention (fish oil vs. placebo).
Measurements: Serum homoarginine, ornithine, citrulline, asymmetric dimethylarginine (ADMA), NG-monomethyl-L-arginine (L-NMMA), and symmetric dimethylarginine (SDMA), maximal voluntary contraction (MVC) and isokinetic torque of the knee extensors at 30° s-1 (MIT), muscle cross sectional area (MCSA) and quality (MQ) were measured at baseline and after the intervention.
Results: No significant exercise-induced changes were observed in metabolite concentrations. There were significant sex differences in the associations between metabolites and muscle parameters. After adjusting for age, glomerular filtration rate and fish oil intervention, citrulline (P=0.002) and ornithine (P=0.022) were negatively associated with MCSA at baseline in males but not females. However, baseline citrulline was negatively correlated with exercise-induced changes in MVC (P=0.043) and MQ (P=0.026) amongst females. Furthermore, amongst males, baseline homoarginine was positively associated with exercise-induced changes in MVC (P=0.026), ADMA was negatively associated with changes in MIT (P=0.026), L-NMMA (p=0.048) and ornithine (P<0.001) were both positively associated with changes in MCSA, and ornithine was negatively associated with changes in MQ (P=0.039).
Conclusion: Therefore, barring citrulline, there are significant sex differences in the associations between L-arginine metabolites and muscle mass and function in healthy older adults. These metabolites might enhance sarcopenia risk stratification, and the success of exercise programs, in old age.
Original languageEnglish
Pages (from-to)534-540
Number of pages7
JournalJournal of Nutrition, Health & Aging
Volume22
Issue number4
Early online date2 Sep 2017
DOIs
Publication statusPublished - 30 Apr 2018

Fingerprint

Sex Characteristics
Arginine
Citrulline
Skeletal Muscle
Ornithine
Exercise
omega-N-Methylarginine
Muscles
Homoarginine
Fish Oils
Placebos
Sarcopenia
Torque
Glomerular Filtration Rate
Knee
Healthy Volunteers
Serum
N,N-dimethylarginine

Keywords

  • L-arginine metabolites
  • muscle mass
  • muscle function
  • old age
  • exercise

Cite this

Sex differences in the associations between L-arginine pathway metabolites, skeletal muscle mass and function, and their responses to resistance exercise, in old age. / Da Boit, Mariasole; Tommasi, S; Elliot, D; Zinellu, A; Sotgia, S; Sibson, R; Meakin, J; Aspden, R M; Carru, Ciriaco; Mangoni, A A; Gray, Stuart R.

In: Journal of Nutrition, Health & Aging, Vol. 22, No. 4, 30.04.2018, p. 534-540.

Research output: Contribution to journalArticle

Da Boit, Mariasole ; Tommasi, S ; Elliot, D ; Zinellu, A ; Sotgia, S ; Sibson, R ; Meakin, J ; Aspden, R M ; Carru, Ciriaco ; Mangoni, A A ; Gray, Stuart R. / Sex differences in the associations between L-arginine pathway metabolites, skeletal muscle mass and function, and their responses to resistance exercise, in old age. In: Journal of Nutrition, Health & Aging. 2018 ; Vol. 22, No. 4. pp. 534-540.
@article{ba131bf665504696b8a55982b3116115,
title = "Sex differences in the associations between L-arginine pathway metabolites, skeletal muscle mass and function, and their responses to resistance exercise, in old age",
abstract = "Objectives: The current study was designed to explore the associations between L-arginine metabolites and muscle mass and function in old age, which are largely unknown.Design: The study used a randomised, double-blind, placebo-controlled design.Setting: The study was carried out in a laboratory setting.Participants: 50 healthy older adults [median age 70 years (IQR 67-73); 27 males].Intervention: Participants undertook an 18-week resistance exercise program, and a nutritional intervention (fish oil vs. placebo).Measurements: Serum homoarginine, ornithine, citrulline, asymmetric dimethylarginine (ADMA), NG-monomethyl-L-arginine (L-NMMA), and symmetric dimethylarginine (SDMA), maximal voluntary contraction (MVC) and isokinetic torque of the knee extensors at 30° s-1 (MIT), muscle cross sectional area (MCSA) and quality (MQ) were measured at baseline and after the intervention.Results: No significant exercise-induced changes were observed in metabolite concentrations. There were significant sex differences in the associations between metabolites and muscle parameters. After adjusting for age, glomerular filtration rate and fish oil intervention, citrulline (P=0.002) and ornithine (P=0.022) were negatively associated with MCSA at baseline in males but not females. However, baseline citrulline was negatively correlated with exercise-induced changes in MVC (P=0.043) and MQ (P=0.026) amongst females. Furthermore, amongst males, baseline homoarginine was positively associated with exercise-induced changes in MVC (P=0.026), ADMA was negatively associated with changes in MIT (P=0.026), L-NMMA (p=0.048) and ornithine (P<0.001) were both positively associated with changes in MCSA, and ornithine was negatively associated with changes in MQ (P=0.039).Conclusion: Therefore, barring citrulline, there are significant sex differences in the associations between L-arginine metabolites and muscle mass and function in healthy older adults. These metabolites might enhance sarcopenia risk stratification, and the success of exercise programs, in old age.",
keywords = "L-arginine metabolites , muscle mass, muscle function , old age, exercise",
author = "{Da Boit}, Mariasole and S Tommasi and D Elliot and A Zinellu and S Sotgia and R Sibson and J Meakin and Aspden, {R M} and Ciriaco Carru and Mangoni, {A A} and Gray, {Stuart R}",
note = "This work was supported by the Biotechnology and Biological Sciences Research Council (BB/J015911/1) and was registered at clinicaltrials.gov (ClinicalTrials. gov Identifier: NCT02843009). Supplementary email included with article",
year = "2018",
month = "4",
day = "30",
doi = "10.1007/s12603-017-0964-6",
language = "English",
volume = "22",
pages = "534--540",
journal = "Journal of Nutrition, Health & Aging",
issn = "1279-7707",
publisher = "Springer Paris",
number = "4",

}

TY - JOUR

T1 - Sex differences in the associations between L-arginine pathway metabolites, skeletal muscle mass and function, and their responses to resistance exercise, in old age

AU - Da Boit, Mariasole

AU - Tommasi, S

AU - Elliot, D

AU - Zinellu, A

AU - Sotgia, S

AU - Sibson, R

AU - Meakin, J

AU - Aspden, R M

AU - Carru, Ciriaco

AU - Mangoni, A A

AU - Gray, Stuart R

N1 - This work was supported by the Biotechnology and Biological Sciences Research Council (BB/J015911/1) and was registered at clinicaltrials.gov (ClinicalTrials. gov Identifier: NCT02843009). Supplementary email included with article

PY - 2018/4/30

Y1 - 2018/4/30

N2 - Objectives: The current study was designed to explore the associations between L-arginine metabolites and muscle mass and function in old age, which are largely unknown.Design: The study used a randomised, double-blind, placebo-controlled design.Setting: The study was carried out in a laboratory setting.Participants: 50 healthy older adults [median age 70 years (IQR 67-73); 27 males].Intervention: Participants undertook an 18-week resistance exercise program, and a nutritional intervention (fish oil vs. placebo).Measurements: Serum homoarginine, ornithine, citrulline, asymmetric dimethylarginine (ADMA), NG-monomethyl-L-arginine (L-NMMA), and symmetric dimethylarginine (SDMA), maximal voluntary contraction (MVC) and isokinetic torque of the knee extensors at 30° s-1 (MIT), muscle cross sectional area (MCSA) and quality (MQ) were measured at baseline and after the intervention.Results: No significant exercise-induced changes were observed in metabolite concentrations. There were significant sex differences in the associations between metabolites and muscle parameters. After adjusting for age, glomerular filtration rate and fish oil intervention, citrulline (P=0.002) and ornithine (P=0.022) were negatively associated with MCSA at baseline in males but not females. However, baseline citrulline was negatively correlated with exercise-induced changes in MVC (P=0.043) and MQ (P=0.026) amongst females. Furthermore, amongst males, baseline homoarginine was positively associated with exercise-induced changes in MVC (P=0.026), ADMA was negatively associated with changes in MIT (P=0.026), L-NMMA (p=0.048) and ornithine (P<0.001) were both positively associated with changes in MCSA, and ornithine was negatively associated with changes in MQ (P=0.039).Conclusion: Therefore, barring citrulline, there are significant sex differences in the associations between L-arginine metabolites and muscle mass and function in healthy older adults. These metabolites might enhance sarcopenia risk stratification, and the success of exercise programs, in old age.

AB - Objectives: The current study was designed to explore the associations between L-arginine metabolites and muscle mass and function in old age, which are largely unknown.Design: The study used a randomised, double-blind, placebo-controlled design.Setting: The study was carried out in a laboratory setting.Participants: 50 healthy older adults [median age 70 years (IQR 67-73); 27 males].Intervention: Participants undertook an 18-week resistance exercise program, and a nutritional intervention (fish oil vs. placebo).Measurements: Serum homoarginine, ornithine, citrulline, asymmetric dimethylarginine (ADMA), NG-monomethyl-L-arginine (L-NMMA), and symmetric dimethylarginine (SDMA), maximal voluntary contraction (MVC) and isokinetic torque of the knee extensors at 30° s-1 (MIT), muscle cross sectional area (MCSA) and quality (MQ) were measured at baseline and after the intervention.Results: No significant exercise-induced changes were observed in metabolite concentrations. There were significant sex differences in the associations between metabolites and muscle parameters. After adjusting for age, glomerular filtration rate and fish oil intervention, citrulline (P=0.002) and ornithine (P=0.022) were negatively associated with MCSA at baseline in males but not females. However, baseline citrulline was negatively correlated with exercise-induced changes in MVC (P=0.043) and MQ (P=0.026) amongst females. Furthermore, amongst males, baseline homoarginine was positively associated with exercise-induced changes in MVC (P=0.026), ADMA was negatively associated with changes in MIT (P=0.026), L-NMMA (p=0.048) and ornithine (P<0.001) were both positively associated with changes in MCSA, and ornithine was negatively associated with changes in MQ (P=0.039).Conclusion: Therefore, barring citrulline, there are significant sex differences in the associations between L-arginine metabolites and muscle mass and function in healthy older adults. These metabolites might enhance sarcopenia risk stratification, and the success of exercise programs, in old age.

KW - L-arginine metabolites

KW - muscle mass

KW - muscle function

KW - old age

KW - exercise

U2 - 10.1007/s12603-017-0964-6

DO - 10.1007/s12603-017-0964-6

M3 - Article

VL - 22

SP - 534

EP - 540

JO - Journal of Nutrition, Health & Aging

JF - Journal of Nutrition, Health & Aging

SN - 1279-7707

IS - 4

ER -