Sheep lumbar intervertebral discs as models for human discs

Janet Elizabeth Reid, Judith Rowena Meakin, S. P. Robins, Janet Mabel Scott Skakle, David William Laurence Hukins

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Objective. To determine the water Content, collagen content and collagen orientation angle in different regions of sheep lumbar discs.

Design. A laboratory study of sheep discs obtained from all abattoir.

Method. A total of 21 sheep lumbar discs were obtained from three lumbar spines. Water content was determined by oven drying (60 degreesC) to constant mass. Collagen content was determined by hydroxyproline analysis. Fibre orientation angles were determined by X-ray diffraction.

Results. Water content increased from 74% of total tissue Mass ill file Outer annulus, to 82% in the inner annulus, to 86% the nucleus. Collagen content decreased from 30% of total tissue mass in the Outer region to 20% in the inner region of the anterior and lateral annulus; it was 16% in the posterior annulus. The orientation angle of the collagen fibres decreased from 59degrees in the outer region to 56degrees in the inner region of the anterior and lateral annulus it was 51degrees in the Posterior annulus.

Conclusions. Sheep lumbar intervertebral discs provide a reasonable model for human lumbar intervertebral discs.

Original languageEnglish
Pages (from-to)312-314
Number of pages2
JournalClinical Biomechanics
Volume17
Issue number4
DOIs
Publication statusPublished - 2002

Keywords

  • collagen
  • intervertebral discs
  • model systems
  • water
  • NUCLEUS PULPOSUS
  • COMPRESSION

Cite this

Sheep lumbar intervertebral discs as models for human discs. / Reid, Janet Elizabeth; Meakin, Judith Rowena; Robins, S. P.; Skakle, Janet Mabel Scott; Hukins, David William Laurence.

In: Clinical Biomechanics, Vol. 17, No. 4, 2002, p. 312-314.

Research output: Contribution to journalArticle

Reid, Janet Elizabeth ; Meakin, Judith Rowena ; Robins, S. P. ; Skakle, Janet Mabel Scott ; Hukins, David William Laurence. / Sheep lumbar intervertebral discs as models for human discs. In: Clinical Biomechanics. 2002 ; Vol. 17, No. 4. pp. 312-314.
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abstract = "Objective. To determine the water Content, collagen content and collagen orientation angle in different regions of sheep lumbar discs.Design. A laboratory study of sheep discs obtained from all abattoir.Method. A total of 21 sheep lumbar discs were obtained from three lumbar spines. Water content was determined by oven drying (60 degreesC) to constant mass. Collagen content was determined by hydroxyproline analysis. Fibre orientation angles were determined by X-ray diffraction.Results. Water content increased from 74{\%} of total tissue Mass ill file Outer annulus, to 82{\%} in the inner annulus, to 86{\%} the nucleus. Collagen content decreased from 30{\%} of total tissue mass in the Outer region to 20{\%} in the inner region of the anterior and lateral annulus; it was 16{\%} in the posterior annulus. The orientation angle of the collagen fibres decreased from 59degrees in the outer region to 56degrees in the inner region of the anterior and lateral annulus it was 51degrees in the Posterior annulus.Conclusions. Sheep lumbar intervertebral discs provide a reasonable model for human lumbar intervertebral discs.",
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T1 - Sheep lumbar intervertebral discs as models for human discs

AU - Reid, Janet Elizabeth

AU - Meakin, Judith Rowena

AU - Robins, S. P.

AU - Skakle, Janet Mabel Scott

AU - Hukins, David William Laurence

PY - 2002

Y1 - 2002

N2 - Objective. To determine the water Content, collagen content and collagen orientation angle in different regions of sheep lumbar discs.Design. A laboratory study of sheep discs obtained from all abattoir.Method. A total of 21 sheep lumbar discs were obtained from three lumbar spines. Water content was determined by oven drying (60 degreesC) to constant mass. Collagen content was determined by hydroxyproline analysis. Fibre orientation angles were determined by X-ray diffraction.Results. Water content increased from 74% of total tissue Mass ill file Outer annulus, to 82% in the inner annulus, to 86% the nucleus. Collagen content decreased from 30% of total tissue mass in the Outer region to 20% in the inner region of the anterior and lateral annulus; it was 16% in the posterior annulus. The orientation angle of the collagen fibres decreased from 59degrees in the outer region to 56degrees in the inner region of the anterior and lateral annulus it was 51degrees in the Posterior annulus.Conclusions. Sheep lumbar intervertebral discs provide a reasonable model for human lumbar intervertebral discs.

AB - Objective. To determine the water Content, collagen content and collagen orientation angle in different regions of sheep lumbar discs.Design. A laboratory study of sheep discs obtained from all abattoir.Method. A total of 21 sheep lumbar discs were obtained from three lumbar spines. Water content was determined by oven drying (60 degreesC) to constant mass. Collagen content was determined by hydroxyproline analysis. Fibre orientation angles were determined by X-ray diffraction.Results. Water content increased from 74% of total tissue Mass ill file Outer annulus, to 82% in the inner annulus, to 86% the nucleus. Collagen content decreased from 30% of total tissue mass in the Outer region to 20% in the inner region of the anterior and lateral annulus; it was 16% in the posterior annulus. The orientation angle of the collagen fibres decreased from 59degrees in the outer region to 56degrees in the inner region of the anterior and lateral annulus it was 51degrees in the Posterior annulus.Conclusions. Sheep lumbar intervertebral discs provide a reasonable model for human lumbar intervertebral discs.

KW - collagen

KW - intervertebral discs

KW - model systems

KW - water

KW - NUCLEUS PULPOSUS

KW - COMPRESSION

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JO - Clinical Biomechanics

JF - Clinical Biomechanics

SN - 0268-0033

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