Abstract
Nonhibernating seasonal mammals have adapted to temporal changes in food availability through behavioral and physiological mechanisms to store food and energy during times of predictable plenty and conserve energy during predicted shortage. Little is known, however, of the hypothalamic neuronal events that lead to a change in behavior or physiology. Here we show for the first time that a shift from long summer-like to short winter-like photoperiod, which induces physiological adaptation to winter in the Siberian hamster, including a body weight decrease of up to 30%, increases neuronal activity in the dorsomedial region of the arcuate nucleus (dmpARC) assessed by electrophysiological patch-clamping recording. Increased neuronal activity in short days is dependent on a photoperiod-driven down-regulation of H3 receptor expression and can be mimicked in long-day dmpARC neurons by the application of the H3 receptor antagonist, cloben-proprit. Short-day activation of dmpARC neurons results in increased c-Fos expression. Tract tracing with the trans-synaptic retrograde tracer, pseudorabies virus, delivered into adipose tissue reveals a multisynaptic neuronal sympathetic outflow from dmpARC to white adipose tissue. These data strongly suggest that increased activity of dmpARC neurons, as a consequence of down-regulation of the histamine H3 receptor, contributes to the physiological adaptation of body weight regulation in seasonal photoperiod. (Endocrinology 150: 3655-3663, 2009)
| Original language | English |
|---|---|
| Pages (from-to) | 3655-3663 |
| Number of pages | 9 |
| Journal | Endocrinology |
| Volume | 150 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - Aug 2009 |
Keywords
- H-3 receptor
- body-weight
- brain histamine
- arcuate nucleus
- energy-balance
- food-intake
- rat-brain
- reproduction
- expression
- system
Cite this
Short Photoperiod-Induced Decrease of Histamine H3 Receptors Facilitates Activation of Hypothalamic Neurons in the Siberian Hamster. / Barrett, Perry; van den Top, M.; Wilson, David; Mercer, Julian; Song, C. K.; Bartness, T. J.; Morgan, Peter John; Spanswick, D.
In: Endocrinology, Vol. 150, No. 8, 08.2009, p. 3655-3663.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Short Photoperiod-Induced Decrease of Histamine H3 Receptors Facilitates Activation of Hypothalamic Neurons in the Siberian Hamster
AU - Barrett, Perry
AU - van den Top, M.
AU - Wilson, David
AU - Mercer, Julian
AU - Song, C. K.
AU - Bartness, T. J.
AU - Morgan, Peter John
AU - Spanswick, D.
PY - 2009/8
Y1 - 2009/8
N2 - Nonhibernating seasonal mammals have adapted to temporal changes in food availability through behavioral and physiological mechanisms to store food and energy during times of predictable plenty and conserve energy during predicted shortage. Little is known, however, of the hypothalamic neuronal events that lead to a change in behavior or physiology. Here we show for the first time that a shift from long summer-like to short winter-like photoperiod, which induces physiological adaptation to winter in the Siberian hamster, including a body weight decrease of up to 30%, increases neuronal activity in the dorsomedial region of the arcuate nucleus (dmpARC) assessed by electrophysiological patch-clamping recording. Increased neuronal activity in short days is dependent on a photoperiod-driven down-regulation of H3 receptor expression and can be mimicked in long-day dmpARC neurons by the application of the H3 receptor antagonist, cloben-proprit. Short-day activation of dmpARC neurons results in increased c-Fos expression. Tract tracing with the trans-synaptic retrograde tracer, pseudorabies virus, delivered into adipose tissue reveals a multisynaptic neuronal sympathetic outflow from dmpARC to white adipose tissue. These data strongly suggest that increased activity of dmpARC neurons, as a consequence of down-regulation of the histamine H3 receptor, contributes to the physiological adaptation of body weight regulation in seasonal photoperiod. (Endocrinology 150: 3655-3663, 2009)
AB - Nonhibernating seasonal mammals have adapted to temporal changes in food availability through behavioral and physiological mechanisms to store food and energy during times of predictable plenty and conserve energy during predicted shortage. Little is known, however, of the hypothalamic neuronal events that lead to a change in behavior or physiology. Here we show for the first time that a shift from long summer-like to short winter-like photoperiod, which induces physiological adaptation to winter in the Siberian hamster, including a body weight decrease of up to 30%, increases neuronal activity in the dorsomedial region of the arcuate nucleus (dmpARC) assessed by electrophysiological patch-clamping recording. Increased neuronal activity in short days is dependent on a photoperiod-driven down-regulation of H3 receptor expression and can be mimicked in long-day dmpARC neurons by the application of the H3 receptor antagonist, cloben-proprit. Short-day activation of dmpARC neurons results in increased c-Fos expression. Tract tracing with the trans-synaptic retrograde tracer, pseudorabies virus, delivered into adipose tissue reveals a multisynaptic neuronal sympathetic outflow from dmpARC to white adipose tissue. These data strongly suggest that increased activity of dmpARC neurons, as a consequence of down-regulation of the histamine H3 receptor, contributes to the physiological adaptation of body weight regulation in seasonal photoperiod. (Endocrinology 150: 3655-3663, 2009)
KW - H-3 receptor
KW - body-weight
KW - brain histamine
KW - arcuate nucleus
KW - energy-balance
KW - food-intake
KW - rat-brain
KW - reproduction
KW - expression
KW - system
U2 - 10.1210/en.2008-1620
DO - 10.1210/en.2008-1620
M3 - Article
VL - 150
SP - 3655
EP - 3663
JO - Endocrinology
JF - Endocrinology
SN - 0013-7227
IS - 8
ER -