Short-term impact of soybean management on ammonia oxidizers in a Brazilian savanna under restoration as revealed by coupling different techniques

E. C P Catão, F. A C Lopes, M. R. Rubini, G. B. Nardoto, J. I. Prosser, R. H. Krüger*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)
4 Downloads (Pure)

Abstract

Interactions between soil characteristics and soil microbiota influence soil ecosystem processes such as nitrification however, their complexity makes interpretation difficult. Furthermore, the impact of soil management systems on abundance and activity of soil microbial community is poorly understood, especially in the Neotropics. To investigate these interactions, the effects of tillage, inorganic fertilization, and plant cover on the abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were assessed by quantification of the marker gene (amoA) during different stages of soybean cultivation in a site under restoration from gravel extraction in the Central Brazilian Savanna (Cerrado). Results of molecular analysis and classic and isotope techniques showed that levels of organic C and NH4 +–N were higher in the soybean field during fallow than in an adjacent undisturbed field (Campo sujo). Ammonia oxidizer abundance and nitrification rates were also higher in the agricultural soil than in the undisturbed site, with the lowest ammonium/nitrate ratio in tilled soil. Soil δ15N was lower in the undisturbed soil than the agricultural soil. Both AOA and AOB were more abundant during soybean crop transitional stages, and this increase positively correlated with soil pH, particularly for AOB abundance, in tilled soil and within the soybean rhizosphere. The results suggest that AOB have more copiotrophic characteristics than AOA and are better able to change available ammonium in the soil. The combination of standard soil ecological methods and modern molecular analysis show the short-term modification of ammonia oxidizer abundance and soil N dynamics in a managed system within the Cerrado biome.

Original languageEnglish
Pages (from-to)401-412
Number of pages12
JournalBiology and Fertility of Soils
Volume52
Issue number3
Early online date19 Jan 2016
DOIs
Publication statusPublished - Apr 2016

Fingerprint

Soybeans
Ammonia
oxidants
savanna
savannas
soybean
ammonia
Soil
soybeans
soil
Archaea
methodology
bacteria
cerrado
agricultural soils
bacterium
nitrification
molecular analysis
Bacteria
agricultural soil

Keywords

  • Ammonia oxidizers
  • amoA
  • Central Brazilian Savanna
  • Cerrado
  • Nitrate
  • Soybean

ASJC Scopus subject areas

  • Soil Science
  • Microbiology
  • Agronomy and Crop Science

Cite this

Short-term impact of soybean management on ammonia oxidizers in a Brazilian savanna under restoration as revealed by coupling different techniques. / Catão, E. C P; Lopes, F. A C; Rubini, M. R.; Nardoto, G. B.; Prosser, J. I.; Krüger, R. H.

In: Biology and Fertility of Soils, Vol. 52, No. 3, 04.2016, p. 401-412.

Research output: Contribution to journalArticle

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N2 - Interactions between soil characteristics and soil microbiota influence soil ecosystem processes such as nitrification however, their complexity makes interpretation difficult. Furthermore, the impact of soil management systems on abundance and activity of soil microbial community is poorly understood, especially in the Neotropics. To investigate these interactions, the effects of tillage, inorganic fertilization, and plant cover on the abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were assessed by quantification of the marker gene (amoA) during different stages of soybean cultivation in a site under restoration from gravel extraction in the Central Brazilian Savanna (Cerrado). Results of molecular analysis and classic and isotope techniques showed that levels of organic C and NH4 +–N were higher in the soybean field during fallow than in an adjacent undisturbed field (Campo sujo). Ammonia oxidizer abundance and nitrification rates were also higher in the agricultural soil than in the undisturbed site, with the lowest ammonium/nitrate ratio in tilled soil. Soil δ15N was lower in the undisturbed soil than the agricultural soil. Both AOA and AOB were more abundant during soybean crop transitional stages, and this increase positively correlated with soil pH, particularly for AOB abundance, in tilled soil and within the soybean rhizosphere. The results suggest that AOB have more copiotrophic characteristics than AOA and are better able to change available ammonium in the soil. The combination of standard soil ecological methods and modern molecular analysis show the short-term modification of ammonia oxidizer abundance and soil N dynamics in a managed system within the Cerrado biome.

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