Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man

P ESSEN, M A MCNURLAN, J WERNERMAN, Eric Milne, E VINNARS, P J GARLICK

    Research output: Contribution to journalArticle

    23 Citations (Scopus)

    Abstract

    The rate of protein synthesis in skeletal muscle was determined in the post-absorptive state and after 3 days of starvation in healthy volunteers. The flooding dose technique employing intravenous injection of (1-C-13)leucine (0.05 g kg-1) was used and incorporation of isotope into muscle protein was measured by taking percutaneous biopsies at 0 and 90 min. Blood samples were taken during the incorporation period for assessment of the enrichment of the free amino acid precursor of protein synthesis. The median (25,75 quartiles) rate of muscle protein synthesis after an overnight fast was 2.03 (2.00,2.23) % days-1 when the precursor enrichment was obtained by measurement of the plasma alpha-ketoisocaproate, taken to be representative of muscle free leucine. Repeat measurements in the same subjects after 3 days of total starvation showed a decrease to 1.82 (1.57,2.05) % days-1. Rates calculated on the basis of the plasma leucine as precursor were 5% lower at both times. An interindividual variation in response to starvation was observed, but the median decrease of 13% in the rate of protein synthesis was statistically significant (P < 0.01).

    Original languageEnglish
    Pages (from-to)287-299
    Number of pages13
    JournalClinical Physiology
    Volume12
    Issue number3
    Publication statusPublished - May 1992

    Keywords

    • Fasting
    • Leucine
    • Protein Synthesis
    • Stable Isotope
    • Amino-Acid Metabolism
    • Turnover
    • Compartmentation
    • <1-C-13>Leucine
    • infusion
    • tissues
    • injury
    • valine
    • liver
    • mass

    Cite this

    ESSEN, P., MCNURLAN, M. A., WERNERMAN, J., Milne, E., VINNARS, E., & GARLICK, P. J. (1992). Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man. Clinical Physiology, 12(3), 287-299.

    Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man. / ESSEN, P ; MCNURLAN, M A ; WERNERMAN, J ; Milne, Eric; VINNARS, E ; GARLICK, P J .

    In: Clinical Physiology, Vol. 12, No. 3, 05.1992, p. 287-299.

    Research output: Contribution to journalArticle

    ESSEN, P, MCNURLAN, MA, WERNERMAN, J, Milne, E, VINNARS, E & GARLICK, PJ 1992, 'Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man', Clinical Physiology, vol. 12, no. 3, pp. 287-299.
    ESSEN P, MCNURLAN MA, WERNERMAN J, Milne E, VINNARS E, GARLICK PJ. Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man. Clinical Physiology. 1992 May;12(3):287-299.
    ESSEN, P ; MCNURLAN, M A ; WERNERMAN, J ; Milne, Eric ; VINNARS, E ; GARLICK, P J . / Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man. In: Clinical Physiology. 1992 ; Vol. 12, No. 3. pp. 287-299.
    @article{50a38cd3b8824df39ad14ec38222194d,
    title = "Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man",
    abstract = "The rate of protein synthesis in skeletal muscle was determined in the post-absorptive state and after 3 days of starvation in healthy volunteers. The flooding dose technique employing intravenous injection of (1-C-13)leucine (0.05 g kg-1) was used and incorporation of isotope into muscle protein was measured by taking percutaneous biopsies at 0 and 90 min. Blood samples were taken during the incorporation period for assessment of the enrichment of the free amino acid precursor of protein synthesis. The median (25,75 quartiles) rate of muscle protein synthesis after an overnight fast was 2.03 (2.00,2.23) {\%} days-1 when the precursor enrichment was obtained by measurement of the plasma alpha-ketoisocaproate, taken to be representative of muscle free leucine. Repeat measurements in the same subjects after 3 days of total starvation showed a decrease to 1.82 (1.57,2.05) {\%} days-1. Rates calculated on the basis of the plasma leucine as precursor were 5{\%} lower at both times. An interindividual variation in response to starvation was observed, but the median decrease of 13{\%} in the rate of protein synthesis was statistically significant (P < 0.01).",
    keywords = "Fasting, Leucine, Protein Synthesis, Stable Isotope, Amino-Acid Metabolism, Turnover, Compartmentation, <1-C-13>Leucine, infusion, tissues, injury, valine, liver, mass",
    author = "P ESSEN and MCNURLAN, {M A} and J WERNERMAN and Eric Milne and E VINNARS and GARLICK, {P J}",
    year = "1992",
    month = "5",
    language = "English",
    volume = "12",
    pages = "287--299",
    journal = "Clinical Physiology",
    issn = "0144-5979",
    publisher = "Wiley-Blackwell",
    number = "3",

    }

    TY - JOUR

    T1 - Short-Term Starvation Decreases Skeletal-Muscle Protein-Synthesis Rate in Man

    AU - ESSEN, P

    AU - MCNURLAN, M A

    AU - WERNERMAN, J

    AU - Milne, Eric

    AU - VINNARS, E

    AU - GARLICK, P J

    PY - 1992/5

    Y1 - 1992/5

    N2 - The rate of protein synthesis in skeletal muscle was determined in the post-absorptive state and after 3 days of starvation in healthy volunteers. The flooding dose technique employing intravenous injection of (1-C-13)leucine (0.05 g kg-1) was used and incorporation of isotope into muscle protein was measured by taking percutaneous biopsies at 0 and 90 min. Blood samples were taken during the incorporation period for assessment of the enrichment of the free amino acid precursor of protein synthesis. The median (25,75 quartiles) rate of muscle protein synthesis after an overnight fast was 2.03 (2.00,2.23) % days-1 when the precursor enrichment was obtained by measurement of the plasma alpha-ketoisocaproate, taken to be representative of muscle free leucine. Repeat measurements in the same subjects after 3 days of total starvation showed a decrease to 1.82 (1.57,2.05) % days-1. Rates calculated on the basis of the plasma leucine as precursor were 5% lower at both times. An interindividual variation in response to starvation was observed, but the median decrease of 13% in the rate of protein synthesis was statistically significant (P < 0.01).

    AB - The rate of protein synthesis in skeletal muscle was determined in the post-absorptive state and after 3 days of starvation in healthy volunteers. The flooding dose technique employing intravenous injection of (1-C-13)leucine (0.05 g kg-1) was used and incorporation of isotope into muscle protein was measured by taking percutaneous biopsies at 0 and 90 min. Blood samples were taken during the incorporation period for assessment of the enrichment of the free amino acid precursor of protein synthesis. The median (25,75 quartiles) rate of muscle protein synthesis after an overnight fast was 2.03 (2.00,2.23) % days-1 when the precursor enrichment was obtained by measurement of the plasma alpha-ketoisocaproate, taken to be representative of muscle free leucine. Repeat measurements in the same subjects after 3 days of total starvation showed a decrease to 1.82 (1.57,2.05) % days-1. Rates calculated on the basis of the plasma leucine as precursor were 5% lower at both times. An interindividual variation in response to starvation was observed, but the median decrease of 13% in the rate of protein synthesis was statistically significant (P < 0.01).

    KW - Fasting

    KW - Leucine

    KW - Protein Synthesis

    KW - Stable Isotope

    KW - Amino-Acid Metabolism

    KW - Turnover

    KW - Compartmentation

    KW - <1-C-13>Leucine

    KW - infusion

    KW - tissues

    KW - injury

    KW - valine

    KW - liver

    KW - mass

    M3 - Article

    VL - 12

    SP - 287

    EP - 299

    JO - Clinical Physiology

    JF - Clinical Physiology

    SN - 0144-5979

    IS - 3

    ER -