Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni

Mariliis Klass, Cornelia Oetke, Leanne E. Lewis, Lars P. Erwig, Astrid P. Heikema, Alistair Easton, Hugh J. Willison, Paul R. Crocker

Research output: Contribution to journalArticle

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Abstract

Sialoadhesin (Sn) is a macrophage (M-phi)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of M-phis with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M-phi interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan–binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-alpha, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-alpha and IFN-beta responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with M-phis in both liver and spleen. In the spleen, IFN-beta–reactive cells were localized to Sn+ M-phis and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.
Original languageEnglish
Pages (from-to)2414-2422
Number of pages9
JournalThe Journal of Immunology
Volume189
Issue number5
Early online date30 Jul 2012
DOIs
Publication statusPublished - 1 Sep 2012

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Sialic Acid Binding Ig-like Lectin 1
Campylobacter jejuni
Macrophages
Spleen
Tumor Necrosis Factor-alpha
Interleukin-12
Phagocytosis
Interleukin-10
Polysaccharides
Immune System

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Klass, M., Oetke, C., Lewis, L. E., Erwig, L. P., Heikema, A. P., Easton, A., ... Crocker, P. R. (2012). Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni. The Journal of Immunology, 189(5), 2414-2422. https://doi.org/10.4049/jimmunol.1200776

Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni. / Klass, Mariliis; Oetke, Cornelia; Lewis, Leanne E.; Erwig, Lars P.; Heikema, Astrid P.; Easton, Alistair; Willison, Hugh J. ; Crocker, Paul R.

In: The Journal of Immunology, Vol. 189, No. 5, 01.09.2012, p. 2414-2422.

Research output: Contribution to journalArticle

Klass, M, Oetke, C, Lewis, LE, Erwig, LP, Heikema, AP, Easton, A, Willison, HJ & Crocker, PR 2012, 'Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni', The Journal of Immunology, vol. 189, no. 5, pp. 2414-2422. https://doi.org/10.4049/jimmunol.1200776
Klass, Mariliis ; Oetke, Cornelia ; Lewis, Leanne E. ; Erwig, Lars P. ; Heikema, Astrid P. ; Easton, Alistair ; Willison, Hugh J. ; Crocker, Paul R. / Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni. In: The Journal of Immunology. 2012 ; Vol. 189, No. 5. pp. 2414-2422.
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