The heterodimeric mycobacterial receptors, macrophage C-type lectin (MCL) and macrophage inducible C-type lectin (Mincle), are upregulated at the cell surface following microbial challenge, but the mechanisms underlying this response are unclear. Here we report that microbial stimulation triggers Mincle expression through the Myeloid differentiation primary response gene 88 (MyD88) pathway; a process that does not require MCL. Conversely, we show that MCL is constitutively expressed but retained intracellularly until Mincle is induced, whereupon the receptors form heterodimers which are translocated to the cell surface. Thus this “two-step” model for induction of these key receptors provides new insights into the underlying mechanisms of anti-mycobacterial immunity.
|Number of pages||5|
|Journal||Microbes and Infection|
|Early online date||19 Mar 2016|
|Publication status||Published - Jul 2016|
- C-type lectin receptor
- TLR signalling
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BD LSR Fortessa
Andrea Holme (Manager)Iain Fraser Cytometry Centre
Research Facilities: Equipment
Iain Fraser Cytometry Centre
Andrea Holme (Manager), Linda Duncan (Senior Application Scientist), Ailsa Laird (Technician) & Kate Burgoyne (Technician)Institute of Medical Sciences
Research Facilities: Facility