Significance of cardiac cannabinoid receptors in regulation of cardiac rhythm, myocardial contractility, and electrophysiologic processes in heart

A. V. Krylatov, L. N. Maslov, S. Yu. Ermakov, O. V. Lasukova, E. I. Barzakh, D. Crawford, R. G. Pertwee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Intravenous administration of cannabinoid (CB) receptor agonists (HU-210, 0.1 mg/kg; ACPA, 0.125 mg/kg; methanandamide, 2.5 mg/kg; and anandamide, 2.5 mg/kg) induced bradycardia in chloralose-anesthetized rats irrespective of the solubilization method. Methanandamide, HU-210, and ACPA had no effect on the electrophysiological activity of the heart, while anandamide increased the duration of the QRS complex. The negative chronotropic effect of HU-210 was due to CB1 receptor activation since it was not observed after CB1 receptor blockade by SR141716A (1 mg/kg intravenously) but was present after pretreatment with CB2 receptor antagonist SR144528 (1 mg/kg intravenously). CB receptor antagonists SR141716A and SR144528 had no effect on cardiac rhythm or ECG indices. Hence, in the intact heart, endogenous CB receptor agonists are not involved in the regulation of cardiac rhythm and electrophysiological processes. The chronotropic effect of CBs was independent of the autonomic nervous system because it remained significant after autonomic ganglion blockade by hexamethonium (1 mg/kg intravenously). CB receptor activation by HU-210 (0.1 and 1 mu M) in vitro decreased the rate and force of isolated heart contractions, the rates of contraction and relaxation, and end diastolic pressure. The negative chronotropic effect of HU-210 was less pronounced in vitro than in vivo. The maximum inotropic effect of HU-210 was reached at the concentration of 0.1 mu M.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalBulletin of Experimental Biology and Medicine
Volume34
Issue number1
DOIs
Publication statusPublished - Jan 2007

Keywords

  • perfused rat-heart
  • international union
  • anesthetized dogs
  • CB1
  • pharmacology
  • delta-9-tetrahydrocannabinol
  • classification
  • constituent
  • performance
  • anandamide

Cite this

Significance of cardiac cannabinoid receptors in regulation of cardiac rhythm, myocardial contractility, and electrophysiologic processes in heart. / Krylatov, A. V.; Maslov, L. N.; Ermakov, S. Yu.; Lasukova, O. V.; Barzakh, E. I.; Crawford, D.; Pertwee, R. G.

In: Bulletin of Experimental Biology and Medicine, Vol. 34, No. 1, 01.2007, p. 28-35.

Research output: Contribution to journalArticle

Krylatov, A. V. ; Maslov, L. N. ; Ermakov, S. Yu. ; Lasukova, O. V. ; Barzakh, E. I. ; Crawford, D. ; Pertwee, R. G. / Significance of cardiac cannabinoid receptors in regulation of cardiac rhythm, myocardial contractility, and electrophysiologic processes in heart. In: Bulletin of Experimental Biology and Medicine. 2007 ; Vol. 34, No. 1. pp. 28-35.
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AU - Crawford, D.

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KW - constituent

KW - performance

KW - anandamide

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