Similarities and differences in surface receptor expression by THP-1 monocytes and differentiated macrophages polarized using seven different conditioning regimens

Megan A. Forrester, Heather J. Wassall, Lindsay S Hall, Huan Cao, Heather M. Wilson, Robert N. Barker, Mark A. Vickers (Corresponding Author)

Research output: Contribution to journalArticle

4 Citations (Scopus)
2 Downloads (Pure)

Abstract

Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.
Original languageEnglish
Pages (from-to)58-76
Number of pages19
JournalCellular Immunology
Volume332
Early online date19 Jul 2018
DOIs
Publication statusPublished - Oct 2018

Fingerprint

Monocytes
Macrophages
Sialic Acid Binding Immunoglobulin-like Lectins
C-Type Lectins
Scavenger Receptors
Macrophage Activation
Flow Cytometry
Phenotype
Cell Line

Keywords

  • Monocyte
  • Macrophage
  • Cell surface
  • Flow cytometry
  • THP-1
  • Polarization

Cite this

@article{d69d89b609ba40b89a0ee61d251528e3,
title = "Similarities and differences in surface receptor expression by THP-1 monocytes and differentiated macrophages polarized using seven different conditioning regimens",
abstract = "Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.",
keywords = "Monocyte, Macrophage, Cell surface, Flow cytometry, THP-1, Polarization",
author = "Forrester, {Megan A.} and Wassall, {Heather J.} and Hall, {Lindsay S} and Huan Cao and Wilson, {Heather M.} and Barker, {Robert N.} and Vickers, {Mark A.}",
note = "We would like to acknowledge the assistance of the Iain Fraser Cytometry Centre at the University of Aberdeen. Funding for this project was provided by the Wellcome Trust (094847).",
year = "2018",
month = "10",
doi = "10.1016/j.cellimm.2018.07.008",
language = "English",
volume = "332",
pages = "58--76",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Similarities and differences in surface receptor expression by THP-1 monocytes and differentiated macrophages polarized using seven different conditioning regimens

AU - Forrester, Megan A.

AU - Wassall, Heather J.

AU - Hall, Lindsay S

AU - Cao, Huan

AU - Wilson, Heather M.

AU - Barker, Robert N.

AU - Vickers, Mark A.

N1 - We would like to acknowledge the assistance of the Iain Fraser Cytometry Centre at the University of Aberdeen. Funding for this project was provided by the Wellcome Trust (094847).

PY - 2018/10

Y1 - 2018/10

N2 - Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.

AB - Macrophages are key in orchestrating immune responses to micro-environmental stimuli, sensed by a complex set of surface receptors. The human cell line THP-1 has a monocytic phenotype, including the ability to differentiate into macrophages, providing a tractable, standardised surrogate for human monocyte-derived macrophages. Here we assessed the expression of 49 surface markers including Fc, complement, C-type lectin and scavenger receptors; TIMs; Siglecs; and co-stimulatory molecules by flow cytometry on both THP-1 monocytes and macrophages and following macrophage activation with seven standard conditioning/polarizing stimuli. Of the 34 surface markers detected on macrophages, 18 altered expression levels on activation. From these, expression of 9 surface markers were consistently altered by all conditioning regimens, while 9 were specific to individual polarizing stimuli. This study provides a resource for the study of macrophages and highlights that macrophage polarization states share much in common and the differences do not easily fit a simple classification system.

KW - Monocyte

KW - Macrophage

KW - Cell surface

KW - Flow cytometry

KW - THP-1

KW - Polarization

U2 - 10.1016/j.cellimm.2018.07.008

DO - 10.1016/j.cellimm.2018.07.008

M3 - Article

C2 - 30077333

VL - 332

SP - 58

EP - 76

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

ER -