Simple assays for measuring innate interactions with fungi

Ann M. Kerrigan, Maria da Glória Teixeira de Sousa, Gordon D. Brown

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Abstract

In recent decades, there has been a steady rise in immunocompromised populations and consequently a dramatic increase in the clinical relevance of normally non-pathogenic and commensal fungi such as Aspergillus fumigatus and Candida albicans. Understanding how these fungi interact with the host immune system is important for the development of immunotherapeutic approaches. Here, we describe a number of methods which have been developed to investigate the interactions of fungi with host leukocytes in vitro, including measuring fungal binding and induction of cytokines, phagocytosis, the respiratory burst, and fungal killing.
Original languageEnglish
Pages (from-to)303-317
Number of pages15
JournalMethods in Molecular Biology
Volume845
DOIs
Publication statusPublished - 2012

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Keywords

  • fungi
  • macrophages
  • phagocytosis
  • respiratory burst
  • thioglycollate
  • cytokine

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