Simultaneous acquisition of absorption and fluorescence spectra of strong absorbers utilizing an evanescent supercontinuum

Johannes Kiefer*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The determination of the absorption and emission spectra of strongly absorbing molecules is challenging, and the data can be biased by self-absorption of the fluorescence signal. To overcome this problem, a total internal reflection approach is proposed. The strongly absorbing sample is placed in an evanescent field of the radiation of a supercontinuum source. The collimated reflected light encodes the absorption spectrum, and the isotropic fluorescence emission is collected in a direction perpendicular to the surface at the same time. This ensures that the emitted light has a minimum possibility of self-absorption inside the sample. (C) 2016 Optical Society of America

Original languageEnglish
Pages (from-to)5684-5687
Number of pages4
JournalOptics Letters
Volume41
Issue number24
Early online date6 Dec 2016
DOIs
Publication statusPublished - 15 Dec 2016

Keywords

  • TOTAL-REFLECTION SPECTROSCOPY
  • OPTICAL WAVE-GUIDE
  • INFRARED-SPECTROSCOPY
  • EMISSION
  • MICROSCOPY
  • WATER

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