Single cell analysis of kynurenine and System L amino acid transport in T cells

Linda V. Sinclair*, Damien Neyens, George Ramsay, Peter M. Taylor, Doreen A. Cantrell

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)
4 Downloads (Pure)

Abstract

The tryptophan metabolite kynurenine has critical immunomodulatory properties and can function as an aryl hydrocarbon receptor (AHR) ligand. Here we show that the ability of T cells to transport kynurenine is restricted to cells activated by the T-cell antigen receptor or proinflammatory cytokines. Kynurenine is transported across the T-cell membrane by the System L transporter SLC7A5. Accordingly, the ability of kynurenine to activate the AHR is restricted to T cells that express SLC7A5. We use the fluorescence spectral properties of kynurenine to develop a flow cytometry-based assay for rapid, sensitive and quantitative measurement of the kynurenine transport capacity in a single cell. Our findings provide a method to assess the susceptibility of T cells to kynurenine, and a sensitive single cell assay to monitor System L amino acid transport.

Original languageEnglish
Article number1981
Number of pages11
JournalNature Communications
Volume9
Early online date17 May 2018
DOIs
Publication statusPublished - 17 May 2018

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Amino Acid Transport System L
Single-Cell Analysis
Kynurenine
T-cells
Systems Analysis
amino acids
T-Lymphocytes
Amino Acids
Large Neutral Amino Acid-Transporter 1
cells
hydrocarbons
Aryl Hydrocarbon Receptors
transporter
cytometry
metabolites
tryptophan
antigens
Assays
Flow cytometry
magnetic permeability

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Single cell analysis of kynurenine and System L amino acid transport in T cells. / Sinclair, Linda V.; Neyens, Damien; Ramsay, George; Taylor, Peter M.; Cantrell, Doreen A.

In: Nature Communications, Vol. 9, 1981, 17.05.2018.

Research output: Contribution to journalArticle

Sinclair, Linda V. ; Neyens, Damien ; Ramsay, George ; Taylor, Peter M. ; Cantrell, Doreen A. / Single cell analysis of kynurenine and System L amino acid transport in T cells. In: Nature Communications. 2018 ; Vol. 9.
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