Single-kernel ionomic profiles are highly heritable indicators of genetic and environmental influences on elemental accumulation in maize grain (Zea mays)

Ivan R Baxter, Gregory Ziegler, Brett Lahner, Michael V. Mickelbart, Rachel Foley, John Danku, Paul Armstrong, David E Salt, Owen A Hoekenga

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Abstract

The ionome, or elemental profile, of a maize kernel can be viewed in at least two distinct ways. First, the collection of elements within the kernel are food and feed for people and animals. Second, the ionome of the kernel represents a developmental end point that can summarize the life history of a plant, combining genetic programs and environmental interactions. We assert that single-kernel-based phenotyping of the ionome is an effective method of analysis, as it represents a reasonable compromise between precision, efficiency, and power. Here, we evaluate potential pitfalls of this sampling strategy using several field-grown maize sample sets. We demonstrate that there is enough genetically determined diversity in accumulation of many of the elements assayed to overcome potential artifacts. Further, we demonstrate that environmental signals are detectable through their influence on the kernel ionome. We conclude that using single kernels as the sampling unit is a valid approach for understanding genetic and environmental effects on the maize kernel ionome.
Original languageEnglish
Article numbere87628
Number of pages10
JournalPloS ONE
Volume9
Issue number1
DOIs
Publication statusPublished - 29 Jan 2014

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Zea mays
Sampling
corn
seeds
Environmental impact
Animals
Artifacts
Food
ionomics
sampling
life history
ionome
phenotype
animals

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Single-kernel ionomic profiles are highly heritable indicators of genetic and environmental influences on elemental accumulation in maize grain (Zea mays). / Baxter, Ivan R; Ziegler, Gregory; Lahner, Brett; Mickelbart, Michael V.; Foley, Rachel; Danku, John; Armstrong, Paul ; Salt, David E; Hoekenga, Owen A.

In: PloS ONE, Vol. 9, No. 1, e87628, 29.01.2014.

Research output: Contribution to journalArticle

Baxter, Ivan R ; Ziegler, Gregory ; Lahner, Brett ; Mickelbart, Michael V. ; Foley, Rachel ; Danku, John ; Armstrong, Paul ; Salt, David E ; Hoekenga, Owen A. / Single-kernel ionomic profiles are highly heritable indicators of genetic and environmental influences on elemental accumulation in maize grain (Zea mays). In: PloS ONE. 2014 ; Vol. 9, No. 1.
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