Skeletal muscle ATP turnover and single fibre ATP and PCr content during intense exercise at different muscle temperatures in humans

Stuart R Gray, Karin Soderlund, Moira Watson, Richard A Ferguson

Research output: Contribution to journalArticle

10 Citations (Scopus)
4 Downloads (Pure)

Abstract

The effect of temperature on skeletal muscle ATP turnover, pulmonary oxygen uptake and single fibre ATP and PCr content was studied during intense cycling exercise. Six healthy male subjects performed 6-min intense (¿50%LT-VO(2peak)) cycling, at 60 rpm, under conditions of normal (N) and elevated muscle temperature (ET). Muscle biopsies obtained from the vastus lateralis at rest, 2 and 6 min were analysed for homogenate ATP, PCr, lactate and glycogen, allowing estimation of anaerobic ATP turnover. Freeze-dried single fibres from biopsies were characterised according to their myosin heavy chain composition (type I, IIA or IIAX) and analysed for ATP and PCr content. Pulmonary gas exchange was measured throughout. There was no difference in pulmonary oxygen uptake between the trials. The elevation of muscle temperature resulted in a lower (P¿
Original languageEnglish
Pages (from-to)885-893
Number of pages9
JournalPflugers Archiv : European Journal of Physiology
Volume462
Issue number6
DOIs
Publication statusPublished - 2011

Fingerprint

Muscle
Skeletal Muscle
Adenosine Triphosphate
Exercise
Muscles
Temperature
Fibers
Biopsy
Oxygen
Pulmonary Gas Exchange
Lung
Myosin Heavy Chains
Quadriceps Muscle
Glycogen
Lactic Acid
Healthy Volunteers
Gases
Chemical analysis

Keywords

  • exercise
  • muscle
  • temperature
  • human muscle
  • metabolism

Cite this

Skeletal muscle ATP turnover and single fibre ATP and PCr content during intense exercise at different muscle temperatures in humans. / Gray, Stuart R; Soderlund, Karin; Watson, Moira; Ferguson, Richard A.

In: Pflugers Archiv : European Journal of Physiology, Vol. 462, No. 6, 2011, p. 885-893.

Research output: Contribution to journalArticle

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