Skeletal muscle myosin heavy chain isoforms and energy metabolism after clenbuterol treatment in the rat

P. Rajab*, J. Fox, S. Riaz, D. Tomlinson, D. Ball, P. L. Greenhaff

*Corresponding author for this work

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48 Citations (Scopus)

Abstract

Prolonged treatment with the β2-adrenoceptor agonist clenbuterol (1-2 mg·kg body mass-1·day -1) is known to induce the hypertrophy of fast-contracting fibers and the conversion of slow- to fast-contracting fibers. We investigated the effects of administering a lower dose of clenbuterol (250 μg·kg body mass-1·day -1) on skeletal muscle myosin heavy chain (MyHC) protein isoform content and adenine nucleotide (ATP, ADP, and AMP) concentrations. Male Wistar rats were administered clenbuterol (n = 8) or saline (n = 6) subcutaneously for 8 wk, after which the extensor digitorum longus (EDL) and soleus muscles were removed. We demonstrated an increase of type IIa MyHC protein content in the soleus from ~0.5% in controls to ~18% after clenbuterol treatment (P < 0.05), which was accompanied by an increase in the total adenine nucleotide pool (TAN; ~19%, P < 0.05) and energy charge [E-C = (ATP + 0.5 ADP)/(ATP + ADP + AMP); ~4%; P < 0.05]. In the EDL, a reduction in the content of the less prevalent type I MyHC protein from ~3% in controls to 0% after clenbuterol treatment (P < 0.05) occurred without any alterations in TAN and E-C. These findings demonstrate that the phenotypic changes previously observed in slow muscle after clenbuterol administration at 1-2 mg·kg body mass-1·day-1 are also observed at a substantially lower dose and are paralleled by concomitant changes in cellular energy metabolism.

Original languageEnglish
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume279
Issue number3 48-3
Publication statusPublished - 2000

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Keywords

  • β-Adrenoceptor agonist
  • Adenosine 5'-triphosphate
  • Energy charge
  • Myosin heavy chain protein composition

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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